ArticlePloS one2007
Whole cell cryo-electron tomography reveals distinct disassembly intermediates of vaccinia virus.
Article in PloS one, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- In situ structure of the poxvirus portal complex.Nature · 2026Article
- Elucidating the protein interaction network of one of the largest icosahedral capsids in the virosphere.The EMBO journal · 2026Article
- Monkeypox Virus Countermeasures: Vaccines, Antibodies, Drugs and Traditional Chinese Medicine.Drug design, development and therapy · 2026Review
- The palisade layer of the poxvirus core is composed of flexible A10 trimers.Nature structural & molecular biology · 2024Article
- 3D Cryo-Correlative Methods to Study Virus Structure and Dynamics Within Cells.Sub-cellular biochemistry · 2024Review
- A succession of two viral lattices drives vaccinia virus assembly.PLoS biology · 2023Article
- Poxvirus under the eyes of electron microscope.Applied microscopy · 2022Review
- Three-dimensional insights into human enveloped viruses in vitro and in situ.Biochemical Society transactions · 2022Review
- Imaging Infection Across Scales of Size: From Whole Animals to Single Molecules.Annual review of microbiology · 2021Review
- A Deleted Deletion Site in a New Vector Strain and Exceptional Genomic Stability of Plaque-Purified Modified Vaccinia Ankara (MVA).Virologica Sinica · 2020Article
- A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles.Scientific reports · 2019Article
- Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication.Frontiers in plant science · 2018Review
- VirusMapper: open-source nanoscale mapping of viral architecture through super-resolution microscopy.Scientific reports · 2016Article
- Viral Infection at High Magnification: 3D Electron Microscopy Methods to Analyze the Architecture of Infected Cells.Viruses · 2015Review
- Fine structure of the vaccinia virion determined by controlled degradation and immunolocalization.Virology · 2015Article
- A cool hybrid approach to the herpesvirus 'life' cycle.Current opinion in virology · 2014Review
- Cryo-electron tomography: the challenge of doing structural biology in situ.The Journal of cell biology · 2013Review
- Multiscale perspectives of virus entry via endocytosis.Virology journal · 2013Review
- Monitoring dynamics of human adenovirus disassembly induced by mechanical fatigue.Scientific reports · 2013Article
- Life cycle of phytoreoviruses visualized by electron microscopy and tomography.Frontiers in microbiology · 2013Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
At each round of infection, viruses fall apart to release their genome for replication, and then reassemble into stable particles within the same host cell. For most viruses, the structural details that underlie these disassembly and assembly reactions are poorly understood. Cryo-electron tomography (cryo-ET), a unique method to investigate large and asymmetric structures at the near molecular resolution, was previously used to study the complex structure of vaccinia virus (VV). Here we study the disassembly of VV by cryo-ET on intact, rapidly frozen, mammalian cells, infected for up to 60 minutes. Binding to the cell surface induced distinct structural rearrangements of the core, such as a shape change, the rearrangement of its surface spikes and de-condensation of the viral DNA. We propose that the cell surface induced changes, in particular the decondensation of the viral genome, are a prerequisite for the subsequent release of the vaccinia DNA into the cytoplasm, which is followed by its cytoplasmic replication. Generally, this is the first study that employs whole cell cryo-ET to address structural details of pathogen-host cell interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.