ArticleBiotechnology and bioengineering2007
Bioluminescence imaging for assessment and normalization in transfected cell arrays.
Article in Biotechnology and bioengineering, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Extracellular Matrix Proteins Confer Cell Adhesion-Mediated Drug Resistance Through Integrin αFrontiers in cell and developmental biology · 2021Article
- Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Article
- Lentiviral arrays for live-cell dynamic monitoring of gene and pathway activity during stem cell differentiation.Molecular therapy : the journal of the American Society of Gene Therapy · 2014Article
- Dynamic transcription factor activity profiling in 2D and 3D cell cultures.Biotechnology and bioengineering · 2013Article
- Fabrication and characterization of DNA-loaded zein nanospheres.Journal of nanobiotechnology · 2012Article
- Optimizing cell arrays for accurate functional genomics.BMC research notes · 2012Article
- The living microarray: a high-throughput platform for measuring transcription dynamics in single cells.BMC genomics · 2011Article
- Cellular arrays for large-scale analysis of transcription factor activity.Biotechnology and bioengineering · 2011Article
- Dynamic, large-scale profiling of transcription factor activity from live cells in 3D culture.PloS one · 2010Article
- Efficacy of immobilized polyplexes and lipoplexes for substrate-mediated gene delivery.Biotechnology and bioengineering · 2009Article
- Functional studies on transfected cell microarray analysed by linear regression modelling.Nucleic acids research · 2008Article
- Surface polyethylene glycol enhances substrate-mediated gene delivery by nonspecifically immobilized complexes.Acta biomaterialia · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Transfected cell arrays (TCAs) represent a high-throughput technique to correlate gene expression with functional cell responses. Despite advances in TCAs, improvements are needed for the widespread application of this technology. We have developed a TCA that combines a two-plasmid system and dual-bioluminescence imaging to quantitatively normalize for variability in transfection and increase sensitivity. The two-plasmids consist of: (i) normalization plasmid present within each spot, and (ii) functional plasmid that varies between spots, responsible for the functional endpoint of the array. Bioluminescence imaging of dual-luciferase reporters (renilla, firefly luciferase) provides sensitive and quantitative detection of cellular response, with minimal post-transfection processing. The array was applied to quantify estrogen receptor alpha (ERalpha) activity in MCF-7 breast cancer cells. A plasmid containing an ERalpha-regulated promoter directing firefly luciferase expression was mixed with a normalization plasmid, complexed with cationic lipids and deposited into an array. ER induction mimicked results obtained through traditional assays methods, with estrogen inducing luciferase expression 10-fold over the antiestrogen fulvestrant or vehicle. Furthermore, the array captured a dose response to estrogen, demonstrating the sensitivity of bioluminescence quantification. This system provides a tool for basic science research, with potential application for the development of patient specific therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.