ArticleProceedings of the National Academy of Sciences of the United States of America2007
Array feature size influences nucleic acid surface capture in DNA microarrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Integration of NanoMIPs With Gold-Patterned Plasmonic Surfaces as Synthetic Protein A.Small science · 2026Article
- Toward the Development of Rapid, Specific, and Sensitive Microfluidic Sensors: A Comprehensive Device Blueprint.JACS Au · 2021Review
- A Reliable, Label Free Quality Control Method for the Production of DNA Microarrays with Clinical Applications.Polymers · 2021Article
- DeepOmix: A scalable and interpretable multi-omics deep learning framework and application in cancer survival analysis.Computational and structural biotechnology journal · 2021Article
- A sensitive DNA capacitive biosensor using interdigitated electrodes.Biosensors & bioelectronics · 2017Article
- Coming of age: ten years of next-generation sequencing technologies.Nature reviews. Genetics · 2016Review
- Photo-attachment of biomolecules for miniaturization on wicking Si-nanowire platform.PloS one · 2015Article
- Evaluating the sensitivity of hybridization-based epigenotyping using a methyl binding domain protein.The Analyst · 2014Article
- BIOPHYSICAL PROPERTIES OF NUCLEIC ACIDS AT SURFACES RELEVANT TO MICROARRAY PERFORMANCE.Biomaterials science · 2014Article
- Drop drying on surfaces determines chemical reactivity - the specific case of immobilization of oligonucleotides on microarrays.BMC biophysics · 2013Article
- Orthogonal patterning of multiple biomolecules using an organic fluorinated resist and imprint lithography.Biomacromolecules · 2013Article
- Signal oscillation is another reason for variability in microarray-based gene expression quantification.PloS one · 2013Article
- Wanted: a positive control for anomalous subdiffusion.Biophysical journal · 2012Review
- High-resolution epifluorescence and time-of-flight secondary ion mass spectrometry chemical imaging comparisons of single DNA microarray spots.Analytical chemistry · 2012Article
- Real-time fluorescent image analysis of DNA spot hybridization kinetics to assess microarray spot heterogeneity.Analytical chemistry · 2012Article
- Size matters: problems and advantages associated with highly miniaturized sensors.Sensors (Basel, Switzerland) · 2012Article
- Aptamer-functionalized microgel particles for protein detection.Analytical chemistry · 2011Article
- Partitioning transcript variation in Drosophila: abundance, isoforms, and alleles.G3 (Bethesda, Md.) · 2011Article
- A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins.The Review of scientific instruments · 2011Article
- Kinetic mechanisms in morpholino-DNA surface hybridization.Journal of the American Chemical Society · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Analyte affinity capture by surface-immobilized diagnostic agents is a routinely used assay format for profiling numerous medically and technologically important target analytes. These assays suffer from numerous performance limitations, including sensitivity and rapidity. Assay miniaturization is advocated to improve surface-capture performance, specifically exploiting the inverse relationship between analyte flux and capture feature size under mass transfer-limiting capture conditions that characterize many such assay formats. Reduced capture feature sizes, e.g., microarrays, are proposed to overcome mass transfer limitations, yet this is difficult to achieve across several size scales. This study validates certain advantages advocated for capture spot miniaturization using a rationale to understand surface capture miniaturization strategies. Experimentally derived immobilized ligand and target capture densities as a function of microspot size for DNA oligomers immobilized on model gold substrates are compared directly with theoretical analysis, validating the hypothesis that miniaturization yields many practical assay advantages. Specifically, results show that transitions from assay mass transfer limiting to kinetically limiting conditions as feature size decreases identify an optimal microspot size range for a specific bioassay system. Analytical advantages realized from such assay miniaturization are more uniform target-spot coverage and substantially increased rate of capture (hybridization), increasing assay signal and rapidity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.