ArticleMolecular and cellular biology2007
Hematopoiesis and thymic apoptosis are not affected by the loss of Cdk2.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- Immunological Phenotyping of Mice with a Point Mutation inBiomedicines · 2023Article
- CD98-induced CD147 signaling stabilizes the Foxp3 protein to maintain tissue homeostasis.Cellular & molecular immunology · 2021Article
- Mechanisms, Hallmarks, and Implications of Stem Cell Quiescence.Stem cell reports · 2019Review
- Targeting cell cycle regulators in hematologic malignancies.Frontiers in cell and developmental biology · 2015Review
- New roles for cyclin-dependent kinases in T cell biology: linking cell division and differentiation.Nature reviews. Immunology · 2014Review
- Article
- Foxp3 protein stability is regulated by cyclin-dependent kinase 2.The Journal of biological chemistry · 2013Article
- Cyclin-dependent kinase 2 controls peripheral immune tolerance.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Less is more: unveiling the functional core of hematopoietic stem cells through knockout mice.Cell stem cell · 2012Review
- Regulation of the g1/s transition in hepatocytes: involvement of the cyclin-dependent kinase cdk1 in the DNA replication.International journal of hepatology · 2012Article
- Cdk2 is required for breast cancer mediated by the low-molecular-weight isoform of cyclin E.Cancer research · 2011Article
- Glycogen synthase kinase-3 plays a central role in mediating glucocorticoid-induced apoptosis.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Coupling of the cell cycle and apoptotic machineries in developing T cells.The Journal of biological chemistry · 2010Article
- Cdk2 plays a critical role in hepatocyte cell cycle progression and survival in the setting of cyclin D1 expression in vivo.Cell cycle (Georgetown, Tex.) · 2009Article
- The cyclin dependent kinase inhibitor (R)-roscovitine prevents alloreactive T cell clonal expansion and protects against acute GvHD.Cell cycle (Georgetown, Tex.) · 2009Article
- The haemopoietic stem cell: between apoptosis and self renewal.The Yale journal of biology and medicine · 2009Review
- Cyclin-dependent kinase 2 signaling regulates myocardial ischemia/reperfusion injury.Journal of molecular and cellular cardiology · 2008Article
- p21 Inhibits Cdk1 in the absence of Cdk2 to maintain the G1/S phase DNA damage checkpoint.Molecular biology of the cell · 2008Article
- The cyclin dependent kinase inhibitor (R)-roscovitine mediates selective suppression of alloreactive human T cells but preserves pathogen-specific and leukemia-specific effectors.Clinical immunology (Orlando, Fla.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Cell cycle regulation is essential for proper homeostasis of hematopoietic cells. Cdk2 is a major regulator of S phase entry, is activated by mitogenic cytokines, and has been suggested to be involved in antigen-induced apoptosis of T lymphocytes. The role of Cdk2 in hematopoietic cells and apoptosis in vivo has not yet been addressed. To determine whether Cdk2 plays a role in these cells, we performed multiple analyses of bone marrow cells, thymocytes, and splenocytes from Cdk2 knockout mice. We found that Cdk2 is not required in vivo to induce apoptosis in lymphocytes, a result that differs from previous pharmacological in vitro studies. Furthermore, thymocyte maturation was not affected by the lack of Cdk2. We then analyzed the hematopoietic stem cell compartment and found similar proportions of stem cells and progenitors in Cdk2(-)(/)(-) and wild-type animals. Knockouts of Cdk2 inhibitors (p21, p27) affect stem cell renewal, but a competitive graft experiment indicated that renewal and multilineage differentiation are normal in the absence of Cdk2. Finally, we stimulated T lymphocytes or macrophages to induce proliferation and observed normal reactivation of Cdk2(-)(/)(-) quiescent cells. Our results indicate that Cdk2 is not required for proliferation and differentiation of hematopoietic cells in vivo, although in vitro analyses consider Cdk2 to be a major player in proliferation and apoptosis in these cells and a potential target for therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.