ReviewMolecular biology reports2008
The use of molecular techniques based on ribosomal RNA and DNA for rumen microbial ecosystem studies: a review.
Review in Molecular biology reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 119 citations in OpenAlex.
- Feedomics provides bidirectional omics strategies between genetics and nutrition for improved production in cattle.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Review
- Investigation of fiber utilization in the rumen of dairy cows based on metagenome-assembled genomes and single-cell RNA sequencing.Microbiome · 2022Article
- A comprehensive update: gastrointestinal microflora, gastric cancer and gastric premalignant condition, and intervention by traditional Chinese medicine.Journal of Zhejiang University. Science. B · 2022Review
- Administration of microencapsulatedFrontiers in veterinary science · 2022Article
- The ever-changing landscape in modern dentistry therapeutics - Enhancing the emptying quiver of the periodontist.Heliyon · 2021Review
- Seasonal Influence on Rumen Microbiota, Rumen Fermentation, and Enteric Methane Emissions of Holstein and Jersey Steers under the Same Total Mixed Ration.Animals : an open access journal from MDPI · 2021Article
- Active Rumen Bacterial and Protozoal Communities Revealed by RNA-Based Amplicon Sequencing on Dairy Cows Fed Different Diets at Three Physiological Stages.Microorganisms · 2021Article
- Metatranscriptomics: an approach for retrieving novel eukaryotic genes from polluted and related environments.3 Biotech · 2020Review
- Effects of Dietary Non-Fibrous Carbohydrate (NFC) to Neutral Detergent Fiber (NDF) Ratio Change on Rumen Bacteria in Sheep Based on Three Generations of Full-Length Amplifiers Sequencing.Animals : an open access journal from MDPI · 2020Article
- A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.Applied microbiology and biotechnology · 2019Article
- Assessment of Rumen Microbiota from a Large Dairy Cattle Cohort Reveals the Pan and Core Bacteriomes Contributing to Varied Phenotypes.Applied and environmental microbiology · 2018Article
- Diarrhea-Associated Intestinal Microbiota in Captive Sichuan Golden Snub-Nosed Monkeys (Rhinopithecus roxellana).Microbes and environments · 2018Article
- Metagenomic investigation of gastrointestinal microbiome in cattle.Asian-Australasian journal of animal sciences · 2017Review
- Isolation and characterization of probiotics from dairies.Iranian journal of microbiology · 2017Article
- Effect of psyllium and gum Arabic biopolymers on the survival rate and storage stability in yogurt ofFood science & nutrition · 2017Article
- Metatranscriptomic Profiling Reveals Linkages between the Active Rumen Microbiome and Feed Efficiency in Beef Cattle.Applied and environmental microbiology · 2017Article
- A practical guide to the oral microbiome and its relation to health and disease.Oral diseases · 2017Review
- Studies on bacterial community composition are affected by the time and storage method of the rumen content.PloS one · 2017Article
- Evaluation of the Bacterial Diversity in the Human Tongue Coating Based on Genus-Specific Primers for 16S rRNA Sequencing.BioMed research international · 2017Article
- Lactobacillus Casei Decreases Organophosphorus Pesticide Diazinon Cytotoxicity in Human HUVEC Cell Line.Advanced pharmaceutical bulletin · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper analyses the research progress in the use of molecular techniques based on ribosomal RNA and DNA (rRNA/rDNA) for rumen microbial ecosystem since first literature by Stahl et al. (1988). Because rumen microbial populations could be under-estimated by adopting the traditional techniques such as roll-tube technique or most-probable-number estimates, modern molecular techniques based on 16S/18S rRNA/rDNA can be used to more accurately provide molecular characterization, microbe populations and classification scheme than traditional methods. Phylogenetic-group-specific probes can be used to hybridize samples for detecting and quantifying of rumen microbes. But, competitive-PCR and real-time PCR can more sensitively quantify rumen microbes than hybridization. Molecular fingerprinting techniques including both denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE) and restriction fragment length polymorphisms (RFLP) can used to explore diversity of bacteria, protozoa and fungi in the rumen ecosystem. By constructing clone libraries of 16S/18S rRNA/rDNA of rumen microbes, more new microbes can be discovered and identified. For fungi, internal transcribed spacers (ITS) of fungi are better than 18S rRNA/rDNA for discriminating operational taxonomic units. In conclusion, 16S/18S rRNA/rDNA procedures have been used with success in rumen microbes and are quickly gaining acceptance for studying rumen microbial ecosystem, and will become useful methods for rumen ecology research. However, molecular techniques based on 16S/18S rRNA/rDNA don't preclude classical and traditional microbiological techniques. It should used together to acquire accurate and satisfactory results.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.