ArticleImmunogenetics2007
Modification of chicken avian beta-defensin-8 at positively selected amino acid sites enhances specific antimicrobial activity.
Article in Immunogenetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- AMPed up immunity: 418 whole genomes reveal intraspecific diversity of koala antimicrobial peptides.Immunogenetics · 2025Article
- Enhancing Antimicrobial Peptide Activity through Modifications of Charge, Hydrophobicity, and Structure.International journal of molecular sciences · 2024Review
- Site Mutation Improves the Expression and Antimicrobial Properties of Fungal Defense.Antibiotics (Basel, Switzerland) · 2023Article
- Pharmacokinetics and Pharmacodynamics of Fungal Defensin NZX AgainstFrontiers in microbiology · 2022Article
- Article
- Developmental and Tissue Patterns of the Basal Expression of Chicken AvianBioMed research international · 2020Article
- Genome-wide analysis reveals the genomic features of the turkey vulture (Cathartes aura) as a scavenger.Molecular genetics and genomics : MGG · 2019Article
- Expression and regulation of avian beta-defensin 8 protein in immune tissues and cell lines of chickens.Asian-Australasian journal of animal sciences · 2018Article
- AvBD1 nucleotide polymorphisms, peptide antimicrobial activities and microbial colonisation of the broiler chicken gut.BMC genomics · 2017Article
- Positive selection in cathelicidin host defense peptides: adaptation to exogenous pathogens or endogenous receptors?Heredity · 2017Article
- Tissue expression and antibacterial activity of host defense peptides in chicken.BMC veterinary research · 2016Article
- Comparative Analysis of the Antimicrobial Activities of Plant Defensin-Like and Ultrashort Peptides against Food-Spoiling Bacteria.Applied and environmental microbiology · 2016Article
- Evolution of the avian β-defensin and cathelicidin genes.BMC evolutionary biology · 2015Article
- Evolution of a cluster of innate immune genes (beta-defensins) along the ancestral lines of chicken and zebra finch.Immunome research · 2010Article
- Comparative in vivo infection models yield insights on early host immune response to Campylobacter in chickens.Immunogenetics · 2009Article
- Molecular diversity of the antimicrobial domain of beta-defensin 3 and homologous peptides.Comparative and functional genomics · 2009Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
Antimicrobial peptides (AMPs), essential components of innate immunity, are found in a range of phylogenetically diverse species and are thought to act by disrupting the membrane integrity of microbes. In this paper, we used evolutionary signatures to identify sites that are most relevant during the functional evolution of these molecules and introduced amino acid substitutions to improve activity. We first demonstrate that the anti-microbial activity of chicken avian beta-defensin-8, previously known as gallinacin-12, can be significantly increased against Escherichia coli, Listeria monocytogenes, Salmonella typhimurium, Salmonella typhimurium phoP- mutant and Streptococcus pyogenes through targeted amino acid substitutions, which confer increased peptide charge. However, by increasing the AMP charge through amino acid substitutions at sites predicted to be subject to positive selection, antimicrobial activity against Escherichia coli was further increased. In contrast, no further increase in activity was observed against the remaining pathogens. This result suggests that charge-increasing modifications confer increased broad-spectrum activity to an AMP, whilst positive selection at particular sites is involved in directing the antimicrobial response against specific pathogens. Thus, there is potential for the rational design of novel therapeutics based on specifically targeted and modified AMPs.
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