ArticleNucleic acids research2007
Untangling the relationships between DNA repair pathways by silencing more than 20 DNA repair genes in human stable clones.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 69 citations in OpenAlex.
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- USP22 Functions as an Oncogenic Driver in Prostate Cancer by Regulating Cell Proliferation and DNA Repair.Cancer research · 2020Article
- Reactive oxygen species levels control NF-κB activation by low dose deferasirox in erythroid progenitors of low risk myelodysplastic syndromes.Oncotarget · 2017Article
- Systematic E2 screening reveals a UBE2D-RNF138-CtIP axis promoting DNA repair.Nature cell biology · 2015Article
- High expression of XPA confers poor prognosis for nasopharyngeal carcinoma patients treated with platinum-based chemoradiotherapy.Oncotarget · 2015Article
- A new phosphorylated form of Ku70 identified in resistant leukemic cells confers fast but unfaithful DNA repair in cancer cell lines.Oncotarget · 2015Article
- Functional Role of NBS1 in Radiation Damage Response and Translesion DNA Synthesis.Biomolecules · 2015Review
- Pyrimidine Pool Disequilibrium Induced by a Cytidine Deaminase Deficiency Inhibits PARP-1 Activity, Leading to the Under Replication of DNA.PLoS genetics · 2015Article
- Reversal of mitochondrial defects with CSB-dependent serine protease inhibitors in patient cells of the progeroid Cockayne syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- PARP-2 depletion results in lower radiation cell survival but cell line-specific differences in poly(ADP-ribose) levels.Cellular and molecular life sciences : CMLS · 2015Article
- Cdk5 promotes DNA replication stress checkpoint activation through RPA-32 phosphorylation, and impacts on metastasis free survival in breast cancer patients.Cell cycle (Georgetown, Tex.) · 2015Article
- Ovarian cancer and DNA repair: DNA ligase IV as a potential key.World journal of clinical oncology · 2013Article
- Overexpressed DNA polymerase iota regulated by JNK/c-Jun contributes to hypermutagenesis in bladder cancer.PloS one · 2013Article
- Article
- Partial complementation of a DNA ligase I deficiency by DNA ligase III and its impact on cell survival and telomere stability in mammalian cells.Cellular and molecular life sciences : CMLS · 2012Article
- Identification of microprocessor-dependent cancer cells allows screening for growth-sustaining micro-RNAs.Oncogene · 2012Article
- Rad51 and DNA-PKcs are involved in the generation of specific telomere aberrations induced by the quadruplex ligand 360A that impair mitotic cell progression and lead to cell death.Cellular and molecular life sciences : CMLS · 2012Article
- Overview of base excision repair biochemistry.Current molecular pharmacology · 2012Review
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Much effort has long been devoted to unraveling the coordinated cellular response to genotoxic insults. In view of the difficulty of obtaining human biological samples of homogeneous origin, I have established a set of stable human clones where one DNA repair gene has been stably silenced by means of RNA interference. I used pEBVsiRNA plasmids that greatly enhance long-term gene silencing in human cells. My older clones reached >500 days in culture. Knock-down HeLa clones maintained a gene silencing phenotype for an extended period in culture, demonstrating that I was able to mimic cells from cancer-prone syndromes. I have silenced >20 genes acting as sensors/transducers (ATM, ATR, Rad50, NBS1, MRE11, PARG and KIN17), or of different DNA repair pathways. In HeLa cells, I have switched off the expression of genes involved in nucleotide excision repair (XPA, XPC, hHR23A, hHR23B, CSA and CSB), nonhomologous end-joining (DNA-PKcs, XRCC4 and Ligase IV), homologous recombination repair (Rad51 and Rad54), or base excision repair (Ogg1 and Ligase III). These cells displayed the expected DNA repair phenotype. We could envisage untangling the complex network between the different DNA repair pathways. In this study, no viral vehicles, with their attendant ethical and safety concerns, were used.
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