ArticleProceedings of the National Academy of Sciences of the United States of America2007
A substrate-independent, 14:3:3 protein-mediated plastid import pathway of NADPH:protochlorophyllide oxidoreductase A.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- The crucial role of mitochondrial/chloroplast-related genes in viral genome replication and host defense: integrative systems biology analysis in plant-virus interaction.Frontiers in microbiology · 2025Article
- Chloroplast: the Trojan horse in plant-virus interaction.Molecular plant pathology · 2018Review
- Cell growth defect factor 1 is crucial for the plastid import of NADPH:protochlorophyllide oxidoreductase A in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- New functions of the chloroplast Preprotein and Amino acid Transporter (PRAT) family members in protein import.Plant signaling & behavior · 2014Review
- Specific targeting of proteins to outer envelope membranes of endosymbiotic organelles, chloroplasts, and mitochondria.Frontiers in plant science · 2014Review
- Comparative analysis of predicted plastid-targeted proteomes of sequenced higher plant genomes.PloS one · 2014Article
- Chaperone receptors: guiding proteins to intracellular compartments.Protoplasma · 2012Review
- Common ground for protein translocation: access control for mitochondria and chloroplasts.Nature reviews. Molecular cell biology · 2011Review
- Etioplast and etio-chloroplast formation under natural conditions: the dark side of chlorophyll biosynthesis in angiosperms.Photosynthesis research · 2010Review
- A search for factors influencing etioplast-chloroplast transition.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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- Recent overview of the Mg branch of the tetrapyrrole biosynthesis leading to chlorophylls.Photosynthesis research · 2008Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastids are semiautonomous organelles that contain only limited coding information in their own DNA. Because most of their genome was transferred to the nucleus after their endosymbiotic origin, plastids must import the major part of their protein constituents from the cytosol. The exact role of cytosolic targeting factors in the regulation of plastid protein import has not been determined. Here, we report that the nucleus-encoded NADPH:protochlorophyllide (Pchlide) oxidoreductase A plastid precursor (pPORA) can use two different plastid import pathways that differ by the requirements for cytosolic 14:3:3 proteins and Hsp70. pPORA synthesized in a wheat germ lysate segregated into different precursor fractions. While import of free pPORA and only Hsp70-complexed pPORA was Pchlide-dependent and involved the previously identified Pchlide-dependent translocon, 14:3:3 protein- and Hsp70-complexed pPORA was transported into Pchlide-free chloroplasts through the Toc75-containing standard translocon at the outer chloroplast membrane/translocon at the inner chloroplast membrane machinery. A 14:3:3 protein binding site was identified in the mature region of the (35)S-pPORA, which governed 14:3:3 protein- and Hsp70-mediated, Pchlide-independent plastid import. Collectively, our results reveal that the import of pPORA into the plastids is tightly regulated and involves different cytosolic targeting factors and plastid envelope translocon complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.