ArticleGenetics2007
Fine-tuning of translation termination efficiency in Saccharomyces cerevisiae involves two factors in close proximity to the exit tunnel of the ribosome.
Article in Genetics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A dual role of the ribosome-bound chaperones RAC/Ssb in maintaining the fidelity of translation termination.Nucleic acids research · 2019Article
- Two chaperones locked in an embrace: structure and function of the ribosome-associated complex RAC.Nature structural & molecular biology · 2017Review
- Article
- Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction.Nature communications · 2016Article
- Release factor eRF3 mediates premature translation termination on polylysine-stalled ribosomes in Saccharomyces cerevisiae.Molecular and cellular biology · 2014Article
- Modulation of stop codon read-through efficiency and its effect on the replication of murine leukemia virus.Journal of virology · 2014Article
- Partial methylation at Am100 in 18S rRNA of baker's yeast reveals ribosome heterogeneity on the level of eukaryotic rRNA modification.PloS one · 2014Article
- Review
- Ribosome profiling reveals pervasive and regulated stop codon readthrough in Drosophila melanogaster.eLife · 2013Article
- The Not4 RING E3 Ligase: A Relevant Player in Cotranslational Quality Control.ISRN molecular biology · 2013Review
- Ribosome-associated complex and Ssb are required for translational repression induced by polylysine segments within nascent chains.Molecular and cellular biology · 2012Article
- Elongation factor eEF1B modulates functions of the release factors eRF1 and eRF3 and the efficiency of translation termination in yeast.BMC molecular biology · 2009Article
- Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1.Nucleic acids research · 2009Article
- Mutants of the Paf1 complex alter phenotypic expression of the yeast prion [PSI+].Molecular biology of the cell · 2009Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In eukaryotes, release factors 1 and 3 (eRF1 and eRF3) are recruited to promote translation termination when a stop codon on the mRNA enters at the ribosomal A-site. However, their overexpression increases termination efficiency only moderately, suggesting that other factors might be involved in the termination process. To determine such unknown components, we performed a genetic screen in Saccharomyces cerevisiae that identified genes increasing termination efficiency when overexpressed. For this purpose, we constructed a dedicated reporter strain in which a leaky stop codon is inserted into the chromosomal copy of the ade2 gene. Twenty-five antisuppressor candidates were identified and characterized for their impact on readthrough. Among them, SSB1 and snR18, two factors close to the exit tunnel of the ribosome, directed the strongest antisuppression effects when overexpressed, showing that they may be involved in fine-tuning of the translation termination level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.