ArticleEukaryotic cell2007
Transgene-induced silencing of the zoosporogenesis-specific NIFC gene cluster of Phytophthora infestans involves chromatin alterations.
Article in Eukaryotic cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 74 citations in OpenAlex.
- Transcriptional programs controlling sporulation in Phytophthora respond to nutrition, humidity, and cryptochrome-mediated light signals.PLoS pathogens · 2026Article
- Pathogenicity and virulence ofVirulence · 2025Review
- RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Signal and regulatory mechanisms involved in spore development ofFrontiers in microbiology · 2022Review
- Biotechnological approaches in management of oomycetes diseases.3 Biotech · 2021Review
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- Chemical genetic approach using β-rubromycin reveals that a RIO kinase-like protein is involved in morphological development in Phytophthora infestans.Scientific reports · 2020Article
- Comparative analyses of saprotrophy in Salisapilia sapeloensis and diverse plant pathogenic oomycetes reveal lifestyle-specific gene expression.FEMS microbiology ecology · 2020Article
- Phytophthora cinnamomi.Molecular plant pathology · 2018Article
- Epigenetic regulation of development and pathogenesis in fungal plant pathogens.Molecular plant pathology · 2017Review
- Article
- The 25-26 nt Small RNAs inFrontiers in microbiology · 2017Article
- Article
- Gene Expression and Silencing Studies in Phytophthora infestans Reveal Infection-Specific Nutrient Transporters and a Role for the Nitrate Reductase Pathway in Plant Pathogenesis.PLoS pathogens · 2016Article
- Diversity, evolution and expression profiles of histone acetyltransferases and deacetylases in oomycetes.BMC genomics · 2016Article
- Diverse Evolutionary Trajectories for Small RNA Biogenesis Genes in the Oomycete Genus Phytophthora.Frontiers in plant science · 2016Article
- Article
- Fragmentation of tRNA in Phytophthora infestans asexual life cycle stages and during host plant infection.BMC microbiology · 2014Article
- Phenotypic diversification by gene silencing in Phytophthora plant pathogens.Communicative & integrative biology · 2013Review
- bZIP transcription factors in the oomycete phytophthora infestans with novel DNA-binding domains are involved in defense against oxidative stress.Eukaryotic cell · 2013Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clustered within the genome of the oomycete phytopathogen Phytophthora infestans are four genes encoding spore-specific nuclear LIM interactor-interacting factors (NIF proteins, a type of transcriptional regulator) that are moderately conserved in DNA sequence. NIFC1, NIFC2, and NIFC3 are zoosporogenesis-induced and grouped within 4 kb, and 20 kb away resides a sporulation-induced form, NIFS. To test the function of the NIFC family, plasmids expressing full-length hairpin constructs of NIFC1 or NIFC2 were stably transformed into P. infestans. This triggered silencing of the cognate gene in about one-third of transformants, and all three NIFC genes were usually cosilenced. However, NIFS escaped silencing despite its high sequence similarity to the NIFC genes. Silencing of the three NIFC genes impaired zoospore cyst germination by 60% but did not affect other aspects of the life cycle. Silencing was transcriptional based on nuclear run-on assays and associated with tighter chromatin packing based on nuclease accessibility experiments. The chromatin alterations extended a few hundred nucleotides beyond the boundaries of the transcribed region of the NIFC cluster and were not associated with increased DNA methylation. A plasmid expressing a short hairpin RNA having sequence similarity only to NIFC1 silenced both that gene and an adjacent member of the gene cluster, likely due to the expansion of a heterochromatic domain from the targeted locus. These data help illuminate the mechanism of silencing in Phytophthora and suggest that caution should be used when interpreting silencing experiments involving closely spaced genes.
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