ArticleApplied and environmental microbiology2007
Simple and rapid F+ coliphage culture, latex agglutination, and typing assay to detect and source track fecal contamination.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 53 citations in OpenAlex.
- Occurrence of coliphage in raw wastewater and in ambient water: A meta-analysis.Water research · 2019Pooled it
- Advancement inBiosensors · 2021Review
- Characterization of a novel group I F-specific RNA bacteriophage isolated from human stool.Archives of microbiology · 2021Article
- Bacteriophages as Fecal Pollution Indicators.Viruses · 2021Review
- Overview of Monitoring Techniques for Evaluating Water Quality at Potable Reuse Treatment Facilities.Journal - American Water Works Association · 2019Article
- Coliphages and Gastrointestinal Illness in Recreational Waters: Pooled Analysis of Six Coastal Beach Cohorts.Epidemiology (Cambridge, Mass.) · 2017Article
- Somatic Coliphage Profiles of Produce and Environmental Samples from Farms in Northern México.Food and environmental virology · 2016Article
- Water quality, weather and environmental factors associated with fecal indicator organism density in beach sand at two recreational marine beaches.The Science of the total environment · 2014Article
- Fecal indicators in sand, sand contact, and risk of enteric illness among beachgoers.Epidemiology (Cambridge, Mass.) · 2012Article
- Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2011Article
- Proposed modifications of Environmental Protection Agency Method 1601 for detection of coliphages in drinking water, with same-day fluorescence-based detection and evaluation by the performance-based measurement system and alternative test protocol validation approaches.Applied and environmental microbiology · 2010Article
- Rapidly measured indicators of recreational water quality and swimming-associated illness at marine beaches: a prospective cohort study.Environmental health : a global access science source · 2010Article
- Presence of enteric viruses in source waters for drinking water production in The Netherlands.Applied and environmental microbiology · 2010Article
- Development of a latex agglutination test for norovirus detection.Journal of microbiology (Seoul, Korea) · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple, rapid, and reliable fecal indicator tests are needed to better monitor and manage ambient waters and treated waters and wastes. Antibody-coated polymeric bead agglutination assays can fulfill these needs and are inexpensive and portable for nonlaboratory settings, and their reagents can be stored at ambient temperatures for months. The goal of this study was to develop, optimize, and validate a rapid microbial water quality monitoring assay using F+ coliphage culture, latex agglutination, and typing (CLAT) to detect F+ coliphage groups with antibody-coated particles. Rapid (180 min) F+ coliphage culture gave comparable results to those with the 16- to 24-h culture time used in EPA method 1601 and was amenable to CLAT assay detection. CLAT was performed on a cardboard card by mixing a drop of coliphage enrichment culture with a drop of antibody-coated polymeric beads as the detection reagent. Visual agglutination or clumping of positive samples occurred in <60 seconds. The CLAT assay had sensitivities of 96.4% (185/192 samples) and 98.2% (161/164 samples) and specificities of 100% (34/34 samples) and 97.7% (129/132 samples) for F+ RNA and DNA coliphages, respectively. CLAT successfully classified F+ RNA coliphages into serogroups typically obtained from human (groups II and III) and animal (groups I and IV) fecal sources, in similar proportions to those obtained with a nucleic acid hybridization assay. This novel group-specific antibody-based particle agglutination technique for rapid and simple detection and grouping of F+ coliphages provides a new and improved tool for monitoring the microbiological quality of drinking, recreational, shellfishing, and other waters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.