ArticleApplied and environmental microbiology2007
Application of flow cytometry to segregated kinetic modeling based on the physiological states of microorganisms.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 49 citations in OpenAlex.
- Physicochemical characteristics and microbial community analysis of a wetland system treating acid mine drainage.Scientific reports · 2025Article
- Combinatorial development of antibacterial Zr-Cu-Al-Ag thin film metallic glasses.Scientific reports · 2016Article
- Evolution of microbiological analytical methods for dairy industry needs.Frontiers in microbiology · 2014Review
- Cell dualism: presence of cells with alternative membrane potentials in growing populations of bacteria and yeasts.Journal of bioenergetics and biomembranes · 2013Article
- Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures.Applied and environmental microbiology · 2010Article
- The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry.Journal of fluorescence · 2010Article
- Detection of infectious adenoviruses in environmental waters by fluorescence-activated cell sorting assay.Applied and environmental microbiology · 2010Article
- Flow cytometry applications in the food industry.Journal of industrial microbiology & biotechnology · 2009Review
- Quantitative approach to determining the contribution of viable-but-nonculturable subpopulations to malolactic fermentation processes.Applied and environmental microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow cytometry (FC) has been introduced to characterize and to assess the physiological states of microorganisms in conjunction with the classical plate-counting method. To show the applicability of the technique, in particular for the development of kinetic models, pure culture fermentation experiments were followed over time, using both prokaryotic (Lactobacillus hilgardii) and eukaryotic (Saccharomyces cerevisiae) microorganisms growing in standard culture media (MRS and YPD). The differences observed between the active and viable cells determined by FC and CFU, respectively, allowed us to determine that a large number of cells were in a viable but nonculturable (VBNC) state, which resulted in a subpopulation much larger than the damaged-cell (double-stained) subpopulation. Finally, the determination of the evolution of viable, the VBNC, and the dead cells allowed us to develop a segregated kinetic model to describe the yeast and the bacteria population dynamics and glucose consumption in batch cultures. This model, more complete than that which is traditionally used, based only on viable cell measurements, describes better the behavior and the functionality of the cultures, giving a deeper knowledge in real time about the status and the course of the bioprocesses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.