ArticleJournal of bacteriology2007
Mutational analysis of the ompA promoter from Flavobacterium johnsoniae.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
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- Systematic dissection of σCell reports · 2021Article
- Colony spreading of the gliding bacterium Flavobacterium johnsoniae in the absence of the motility adhesin SprB.Scientific reports · 2021Article
- Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.Nucleic acids research · 2021Article
- Regulation of alginate catabolism involves a GntR family repressor in the marine flavobacterium Zobellia galactanivorans DsijT.Nucleic acids research · 2020Article
- Global analysis of protein synthesis in Flavobacterium johnsoniae reveals the use of Kozak-like sequences in diverse bacteria.Nucleic acids research · 2019Article
- An Extracytoplasmic Function Sigma Factor Controls Cellulose Utilization by Regulating the Expression of an Outer Membrane Protein inApplied and environmental microbiology · 2019Article
- Differential RNA Sequencing Implicates Sulfide as the Master Regulator of SApplied and environmental microbiology · 2018Article
- A Putative Type II Secretion System Is Involved in Cellulose Utilization inFrontiers in microbiology · 2017Article
- The Emerging Fish PathogenFrontiers in microbiology · 2017Article
- The Role of the Regulator Fur in Gene Regulation and Virulence ofFrontiers in cellular and infection microbiology · 2017Article
- cis-Encoded Small RNAs, a Conserved Mechanism for Repression of Polysaccharide Utilization in Bacteroides.Journal of bacteriology · 2016Article
- The mannitol utilization system of the marine bacterium Zobellia galactanivorans.Applied and environmental microbiology · 2015Article
- Elizabethkingia anophelis: molecular manipulation and interactions with mosquito hosts.Applied and environmental microbiology · 2015Article
- Identification of a 4-deoxy-L-erythro-5-hexoseulose uronic acid reductase, FlRed, in an alginolytic bacterium Flavobacterium sp. strain UMI-01.Marine drugs · 2015Article
- Flavobacterium johnsoniae PorV is required for secretion of a subset of proteins targeted to the type IX secretion system.Journal of bacteriology · 2015Article
- FLP-FRT-based method to obtain unmarked deletions of CHU_3237 (porU) and large genomic fragments of Cytophaga hutchinsonii.Applied and environmental microbiology · 2014Article
- Novel outer membrane protein involved in cellulose and cellooligosaccharide degradation by Cytophaga hutchinsonii.Applied and environmental microbiology · 2014Article
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5 authors.
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Abstract
Sequences that mediate the initiation of transcription in Flavobacterium species are not well known. The majority of identified Flavobacterium promoter elements show homology to those of other members of the phylum Bacteroidetes, but not of proteobacteria, and they function poorly in Escherichia coli. In order to analyze the Flavobacterium promoter structure systematically, we investigated the -33 consensus element, -7 consensus element, and spacer length of the Flavobacterium ompA promoter by measuring the effects of site-directed mutations on promoter activity. The nonconserved sequences in the spacer region and in regions close to the consensus motifs were randomized in order to determine their importance for promoter activity. Most of the base substitutions in these regions caused large decreases in promoter activity. The optimal -33/-7 motifs (TTTG/TANNTTTG) were identical to Bacteroides fragilis sigma(ABfr) consensus -33/-7 promoter elements but lacked similarity to the E. coli sigma(70) promoter elements. The length of the spacer separating the -33 and -7 motifs of the ompA promoter also had a pronounced effect on promoter activity, with 19 bp being optimal. In addition to the consensus promoter elements and spacer length, the GC content of the core promoter sequences had a pronounced effect on Flavobacterium promoter activity. This information was used to conduct a scan of the Flavobacterium johnsoniae and B. fragilis genomes for putative promoters, resulting in 188 hits in B. fragilis and 109 hits in F. johnsoniae.
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