ArticleBiophysical journal2007
Two-dimensional standing wave total internal reflection fluorescence microscopy: superresolution imaging of single molecular and biological specimens.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging.Light, science & applications · 2024Article
- Total Internal Reflection Fluorescence (TIRF) Microscopy.Current protocols · 2022Review
- Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking.International journal of molecular sciences · 2022Article
- Super-Resolution Live Cell Microscopy of Membrane-Proximal Fluorophores.International journal of molecular sciences · 2020Article
- Fluorescence imaging with tailored light.Nanophotonics (Berlin, Germany) · 2019Article
- Inverse matrix based phase estimation algorithm for structured illumination microscopy.Biomedical optics express · 2018Article
- Single-shot super-resolution total internal reflection fluorescence microscopy.Nature methods · 2018Article
- Three-dimensional wide-field pump-probe structured illumination microscopy.Optics express · 2017Article
- Spatiotemporal Fluctuation Analysis: A Powerful Tool for the Future Nanoscopy of Molecular Processes.Biophysical journal · 2016Article
- Does super-resolution fluorescence microscopy obsolete previous microscopic approaches to protein co-localization?Methods in molecular biology (Clifton, N.J.) · 2015Review
- Wide-field fluorescent microscopy and fluorescent imaging flow cytometry on a cell-phone.Journal of visualized experiments : JoVE · 2013Article
- Single cell optical imaging and spectroscopy.Chemical reviews · 2013Review
- Lens-free computational imaging of capillary morphogenesis within three-dimensional substrates.Journal of biomedical optics · 2012Article
- Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Lensfree super-resolution holographic microscopy using wetting films on a chip.Optics express · 2011Article
- Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications.Lab on a chip · 2010Article
- Total internal reflection fluorescence (TIRF) microscopy.Current protocols in cytometry · 2009Review
- Fluorescence microscopy below the diffraction limit.Seminars in cell & developmental biology · 2009Review
- Wide-field extended-resolution fluorescence microscopy with standing surface-plasmon-resonance waves.Optics letters · 2009Article
- Super-resolution video microscopy of live cells by structured illumination.Nature methods · 2009Article
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5 authors.
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Abstract
The development of high resolution, high speed imaging techniques allows the study of dynamical processes in biological systems. Lateral resolution improvement of up to a factor of 2 has been achieved using structured illumination. In a total internal reflection fluorescence microscope, an evanescence excitation field is formed as light is total internally reflected at an interface between a high and a low index medium. The <100 nm penetration depth of evanescence field ensures a thin excitation region resulting in low background fluorescence. We present even higher resolution wide-field biological imaging by use of standing wave total internal reflection fluorescence (SW-TIRF). Evanescent standing wave (SW) illumination is used to generate a sinusoidal high spatial frequency fringe pattern on specimen for lateral resolution enhancement. To prevent thermal drift of the SW, novel detection and estimation of the SW phase with real-time feedback control is devised for the stabilization and control of the fringe phase. SW-TIRF is a wide-field superresolution technique with resolution better than a fifth of emission wavelength or approximately 100 nm lateral resolution. We demonstrate the performance of the SW-TIRF microscopy using one- and two-directional SW illumination with a biological sample of cellular actin cytoskeleton of mouse fibroblast cells as well as single semiconductor nanocrystal molecules. The results confirm the superior resolution of SW-TIRF in addition to the merit of a high signal/background ratio from TIRF microscopy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.