ArticleBiophysical journal2007
A kinetic model for calcium dynamics in RAW 264.7 cells: 1. Mechanisms, parameters, and subpopulational variability.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
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- Mechanisms underlying divergent relationships between CaThe Journal of physiology · 2023Article
- Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals.Micromachines · 2021Article
- Dendritic spine geometry and spine apparatus organization govern the spatiotemporal dynamics of calcium.The Journal of general physiology · 2019Article
- Two independent but synchronized Gβγ subunit-controlled pathways are essential for trailing-edge retraction during macrophage migration.The Journal of biological chemistry · 2017Article
- Signal Transduction at the Single-Cell Level: Approaches to Study the Dynamic Nature of Signaling Networks.Journal of molecular biology · 2016Review
- Endothelial cells decode VEGF-mediated Ca2+ signaling patterns to produce distinct functional responses.Science signaling · 2016Article
- Calcium Dynamics of Ex Vivo Long-Term Cultured CD8+ T Cells Are Regulated by Changes in Redox Metabolism.PloS one · 2016Article
- Immobilized IL-8 Triggers Phagocytosis and Dynamic Changes in Membrane Microtopology in Human Neutrophils.Annals of biomedical engineering · 2015Article
- Microfluidic interrogation and mathematical modeling of multi-regime calcium signaling dynamics.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Functional classification of skeletal muscle networks. I. Normal physiology.Journal of applied physiology (Bethesda, Md. : 1985) · 2012Article
- An integrated stochastic model of "inside-out" integrin activation and selective T-lymphocyte recruitment.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Bioinformatics and systems biology of the lipidome.Chemical reviews · 2011Review
- Integration of lipidomics and transcriptomics data towards a systems biology model of sphingolipid metabolism.BMC systems biology · 2011Article
- High-throughput analysis of calcium signalling kinetics in astrocytes stimulated with different neurotransmitters.PloS one · 2011Article
- Stochastic hybrid modeling of intracellular calcium dynamics.The Journal of chemical physics · 2010Article
- Variability in G-protein-coupled signaling studied with microfluidic devices.Biophysical journal · 2010Article
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2 authors at 1 institution in 1 country.
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Abstract
Calcium (Ca(2+)) is an important second messenger and has been the subject of numerous experimental measurements and mechanistic studies in intracellular signaling. Calcium profile can also serve as a useful cellular phenotype. Kinetic models of calcium dynamics provide quantitative insights into the calcium signaling networks. We report here the development of a complex kinetic model for calcium dynamics in RAW 264.7 cells stimulated by the C5a ligand. The model is developed using the vast number of measurements of in vivo calcium dynamics carried out in the Alliance for Cellular Signaling (AfCS) Laboratories. Ligand binding, phospholipase C-beta (PLC-beta) activation, inositol 1,4,5-trisphosphate (IP(3)) receptor (IP(3)R) dynamics, and calcium exchange with mitochondria and extracellular matrix have all been incorporated into the model. The experimental data include data from both native and knockdown cell lines. Subpopulational variability in measurements is addressed by allowing nonkinetic parameters to vary across datasets. The model predicts temporal response of Ca(2+) concentration for various doses of C5a under different initial conditions. The optimized parameters for IP(3)R dynamics are in agreement with the legacy data. Further, the half-maximal effect concentration of C5a and the predicted dose response are comparable to those seen in AfCS measurements. Sensitivity analysis shows that the model is robust to parametric perturbations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.