ArticleBiophysical journal2007
Single-particle tracking of membrane protein diffusion in a potential: simulation, detection, and application to confined diffusion of CFTR Cl- channels.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imaging.Cell · 2026Article
- Machine Learning in Single-Molecule Tracking Analysis of Superresolution Optical Microscopy Data.Cells · 2026Review
- Feature Selection and Hyperparameter Optimization for Machine Learned Classification of 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells.Nature methods · 2025Article
- Single molecule dynamics in a virtual cell combining a 3-dimensional matrix model with random walks.Scientific reports · 2024Article
- A Proteomic Survey of the Cystic Fibrosis Transmembrane Conductance Regulator Surfaceome.International journal of molecular sciences · 2023Review
- Extending resolution within a single imaging frame.Nature communications · 2022Article
- Three-dimensional localization refinement and motion model parameter estimation for confined single particle tracking under low-light conditions.Biomedical optics express · 2021Article
- Single-particle diffusional fingerprinting: A machine-learning framework for quantitative analysis of heterogeneous diffusion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Surface-modified elastomeric nanofluidic devices for single nanoparticle trapping.Microsystems & nanoengineering · 2021Article
- GM1 as Adjuvant of Innovative Therapies for Cystic Fibrosis Disease.International journal of molecular sciences · 2020Article
- Understanding biochemical processes in the presence of sub-diffusive behavior of biomolecules in solution and living cells.Biophysical reviews · 2019Review
- Influenza Hemagglutinin Modulates Phosphatidylinositol 4,5-Bisphosphate Membrane Clustering.Biophysical journal · 2019Article
- Detection of Velocity and Diffusion Coefficient Change Points in Single-Particle Trajectories.Biophysical journal · 2018Article
- Errors in Energy Landscapes Measured with Particle Tracking.Biophysical journal · 2018Article
- Effect of fish oil on lateral mobility of prostaglandin FDomestic animal endocrinology · 2017Article
- Perspective: On the importance of hydrodynamic interactions in the subcellular dynamics of macromolecules.The Journal of chemical physics · 2016Article
- ATP-Binding Cassette Transporter Structure Changes Detected by Intramolecular Fluorescence Energy Transfer for High-Throughput Screening.Molecular pharmacology · 2015Article
- PKA and actin play critical roles as downstream effectors in MRP4-mediated regulation of fibroblast migration.Cellular signalling · 2015Article
Corrections and comments
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Authors and funding
3 authors.
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Abstract
Confined diffusion of membrane receptors and lipids can result from intramembrane barriers, skeletal interactions, rafts, and other phenomena. We simulated single-particle diffusion in two dimensions in an arbitrary potential, V(r), based on summation of random and potential gradient-driven motions. Algorithms were applied and verified for detection of potential-driven diffusion, and for determination of V(r) from radial particle density distributions, taking into account experimental uncertainties in particle position and finite trajectory recording. Single-particle tracking (SPT) analysis of the diffusion of cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channels in mammalian cells revealed confined diffusion with diffusion coefficient approximately 0.004 microm(2)/s. SPT data fitted closely to a springlike attractive potential, V(r) = kr(2), but not to other V(r) forms such as hard-wall or viscoelastic-like potentials. The "spring constant", k, determined from SPT data was 2.6 +/- 0.8 pN/microm, and not altered significantly by modulation of skeletal protein architecture by jasplakinolide. However, k was reduced by a low concentration of latrunculin, supporting the involvement of actin in the springlike tethering of CFTR. Confined diffusion of membrane proteins is likely a general phenomenon suitable for noninvasive V(r) analysis of force-producing mechanisms. Our data provide the first measurement of actin elasticity, to the best of our knowledge, that does not involve application of an external force.
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Registered trials
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