ArticleNucleic acids research2007
Sequences in attB that affect the ability of phiC31 integrase to synapse and to activate DNA cleavage.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
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- Complete genomes and comparative analyses of Streptomyces phages that influence secondary metabolism and sporulation.Scientific reports · 2023Article
- A bipartite thermodynamic-kinetic contribution by an activating mutation to RDF-independent excision by a phage serine integrase.Nucleic acids research · 2020Article
- Serine Integrase attP Binding and Specificity.Journal of molecular biology · 2018Article
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- Genome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe.Applied and environmental microbiology · 2017Article
- Single-molecule analysis of ϕC31 integrase-mediated site-specific recombination by tethered particle motion.Nucleic acids research · 2016Article
- The ins and outs of serine integrase site-specific recombination.Current opinion in structural biology · 2014Review
- Cross-talk between diverse serine integrases.Journal of molecular biology · 2014Article
- Attachment site recognition and regulation of directionality by the serine integrases.Nucleic acids research · 2013Article
- Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination.PLoS genetics · 2013Article
- The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase.Mobile DNA · 2013Article
- Adjusting the attB site in donor plasmid improves the efficiency of ΦC31 integrase system.DNA and cell biology · 2012Article
- Characterization of DNA sequences required for the CcrAB-mediated integration of staphylococcal cassette chromosome mec, a Staphylococcus aureus genomic island.Journal of bacteriology · 2012Article
- Toward improvement of erythromycin A production in an industrial Saccharopolyspora erythraea strain via facilitation of genetic manipulation with an artificial attB site for specific recombination.Applied and environmental microbiology · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Phage integrases are required for recombination of the phage genome with the host chromosome either to establish or exit from the lysogenic state. C31 integrase is a member of the serine recombinase family of site-specific recombinases. In the absence of any accessory factors integrase is unidirectional, catalysing the integration reaction between the phage and host attachment sites, attP x attB to generate the hybrid sites, attL and attR. The basis for this directionality is due to selective synapsis of attP and attB sites. Here we show that mutations in attB can block the integration reaction at different stages. Mutations at positions distal to the crossover site inhibit recombination by destabilizing the synapse with attP without significantly affecting DNA-binding affinity. These data are consistent with the proposal that integrase adopts a specific conformation on binding to attB that permits synapsis with attP. Other attB mutants with changes close to the crossover site are able to form a stable synapse but cleavage of the substrates is prevented. These mutants indicate that there is a post-synaptic DNA recognition event that results in activation of DNA cleavage.
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