ArticleNucleic acids research2007
On the stability of peptide nucleic acid duplexes in the presence of organic solvents.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- RNA detection on a microfluidic platform using Thyclotides.Sensors and actuators reports · 2026Article
- Long-Term Stabilization and Storage of Peptide-Based Coacervate through Tyrosine-Rich Sequences and Polyphenol Network.Nano letters · 2025Article
- Shorter Peptide Nucleic Acid Probes Improve Affibody-Mediated Peptide Nucleic Acid-Based Pretargeting.ACS pharmacology & translational science · 2024Article
- Supramolecular Nucleic Acid-Based Organosilica Nanoparticles Responsive to Physical and Biological Inputs.Journal of the American Chemical Society · 2023Article
- Responsive Nucleic Acid-Based Organosilica Nanoparticles.Journal of the American Chemical Society · 2023Article
- A red light-triggered chemical tool for sequence-specific alkylation of G-quadruplex and I-motif DNA.Nucleic acids research · 2023Article
- Modeling Peptide Nucleic Acid Binding Enthalpies Using MM-GBSA.The journal of physical chemistry. B · 2022Article
- Synthesis and structure-activity relationship of peptide nucleic acid probes with improved interstrand-crosslinking abilities: application to biotin-mediated RNA-pulldown.RSC chemical biology · 2022Article
- Development of an Automatable Affinity Purification Process for DNA-Encoded Chemistry.ACS omega · 2022Article
- Nucleic Acid Identity, Structure, and Flexibility Affect the Electrochemical Signal of Tethered Redox Molecules upon Biopolymer Collapse.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Apolar chemical environments compact unfolded RNAs and can promote folding.Biophysical reports · 2021Article
- Modular self-assembly of gamma-modified peptide nucleic acids in organic solvent mixtures.Nature communications · 2020Article
- Thermal Stability of RNA Structures with Bulky Cations in Mixed Aqueous Solutions.Biophysical journal · 2016Article
- The structural stability and catalytic activity of DNA and RNA oligonucleotides in the presence of organic solvents.Biophysical reviews · 2016Review
- Enhanced deoxyribozyme-catalyzed RNA ligation in the presence of organic cosolvents.Biopolymers · 2013Article
- Hydration changes upon DNA folding studied by osmotic stress experiments.Biophysical journal · 2012Article
- Molecular computing by PNA:PNA duplex formation.Artificial DNA, PNA & XNA · 2011Article
- Comparative genome-wide analysis of small RNAs of major Gram-positive pathogens: from identification to application.Microbial biotechnology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid double helices are stabilized by hydrogen bonding and stacking forces (a combination of hydrophobic, dispersive and electrostatic forces) of the base pairs in the helix. One would predict the hydrogen bonding contributions to increase and the stacking contributions to decrease as the water activity in the medium decreases. Study of nucleobase paired duplexes in the absence of water and ultimately in pure aprotic, non-polar organic solvents is not possible with natural phosphodiester nucleic acids due to the ionic phosphate groups and the associated cations, but could be possible with non-ionic nucleic acid analogues or mimics such as peptide nucleic acids. We now report that peptide nucleic acid (PNA) (in contrast to DNA) duplexes show almost unaffected stability in up to 70% dimethylformamide (DMF) or dioxane, and extrapolation of the data to conditions of 100% organic solvents indicates only minor (or no) destabilization of the PNA duplexes. Our data indicate that stacking forces contribute little if at all to the duplex stability under these conditions. The differences in behaviour between the PNA and the DNA duplexes are attributed to the differences in hydration and counter ion release rather than to the differences in nucleobase interaction. These results support the possibility of having stable nucleobase paired double helices in organic solvents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.