ReviewJournal of industrial microbiology & biotechnology2007
Molecular approaches: advantages and artifacts in assessing bacterial diversity.
Review in Journal of industrial microbiology & biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed.
- Characterization of symbiotic and nitrogen fixing bacteria.AMB Express · 2022Review
- Current Classification of the Bacillus pumilus Group Species, the Rubber-Pathogenic Bacteria Causing Trunk Bulges Disease in Malaysia as Assessed by MLSA and Multi rep-PCR Approaches.The plant pathology journal · 2021Article
- Evaluation of 16S rRNA Hypervariable Regions for Bioweapon Species Detection by Massively Parallel Sequencing.International journal of microbiology · 2020Article
- Identification and Classification for theFrontiers in microbiology · 2018Article
- The role of probiotics, prebiotics and synbiotics in animal nutrition.Gut pathogens · 2018Review
- A novel rat model of catheter-associated urinary tract infection.International urology and nephrology · 2015Article
- rpoD gene pyrosequencing for the assessment of Pseudomonas diversity in a water sample from the Woluwe River.Applied and environmental microbiology · 2014Article
- Virio- and bacterioplankton microscale distributions at the sediment-water interface.PloS one · 2014Article
- Article
- Promiscuity in mice is associated with increased vaginal bacterial diversity.Die Naturwissenschaften · 2011Article
- Comparison of Phenotypic and Genotypic Methods Used for the Species Identification of Lactobacillus NP51 and Development of a Strain-Specific PCR Assay.Probiotics and antimicrobial proteins · 2010Article
- Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA.Journal of microbiology (Seoul, Korea) · 2009Article
- Genotypic and phenotypic diversity of Bacillus spp. isolated from steel plant waste.BMC research notes · 2008Article
- A comparison of methods for total community DNA preservation and extraction from various thermal environments.Journal of industrial microbiology & biotechnology · 2008Article
- Archaeal diversity in a Fe-As rich acid mine drainage at Carnoulès (France).Extremophiles : life under extreme conditions · 2008Article
- A biological treasure metagenome: pave a way for big science.Indian journal of microbiology · 2008Article
- Microbial diversity and genomics in aid of bioenergy.Journal of industrial microbiology & biotechnology · 2008Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria account for a major proportion of Earth's biological diversity. They play essential roles in quite diverse environments and there has been an increasing interest in bacterial biodiversity. Research using novel and efficient tools to identify and characterize bacterial communities has been the key for elucidating biological activities with potential for industrial application. The current approach used for defining bacterial species is based on phenotypic and genomic properties. Traditional and novel DNA-based molecular methods are improving our knowledge of bacterial diversity in nature. Advances in molecular biology have been important for studies of diversity, considerably improving our knowledge of morphological, physiological, and ecological features of bacterial taxa. DNA-DNA hybridization, which has been used for many years, is still considered the golden standard for bacteria species identification. PCR-based methods investigating 16S rRNA gene sequences, and other approaches, such as the metagenome, have been used to study the physiology and diversity of bacteria and to identify novel genes with potential pharmaceutical and other biotechnological applications. We examined the advantages and limitations of molecular methods currently used to analyze bacterial diversity; these are mainly based on the 16S rRNA gene. These methods have allowed us to examine microorganisms that cannot be cultivated by routine methods and have also been useful for phylogenetic studies. We also considered the importance of improvements in microbe culture techniques and how we can combine different methods to allow a more appropriate assessment of bacterial diversity and to determine their real potential for industrial applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.