ArticleJournal of the American Society of Nephrology : JASN2007
Mouse embryonic stem cell-derived embryoid bodies generate progenitors that integrate long term into renal proximal tubules in vivo.
Article in Journal of the American Society of Nephrology : JASN, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 158 citations in OpenAlex.
- The effect of adipose-derived stem cells (ADSC) treatment on kidney histopathological appearance on the Wistar rat models with grade five kidney trauma.Innovative surgical sciences · 2024Article
- Adult Mouse Kidney Stem Cells Orchestrate the De Novo Assembly of a Nephron via Sirt2-Modulated Canonical Wnt/β-Catenin Signaling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Assessing kidney development and disease using kidney organoids and CRISPR engineering.Frontiers in cell and developmental biology · 2022Review
- Human reconstructed kidney models.In vitro cellular & developmental biology. Animal · 2021Review
- Advances in understanding vertebrate nephrogenesis.Tissue barriers · 2020Article
- Recapitulating kidney development in vitro by priming and differentiating mouse embryonic stem cells in monolayers.NPJ Regenerative medicine · 2020Article
- Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis.Scientific reports · 2018Article
- Functional comparison of distinctBiology open · 2018Article
- Renal lineage cells as a source for renal regeneration.Pediatric research · 2018Review
- Concise Review: Kidney Generation with Human Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2017Review
- Kidney Organoids: A Translational Journey.Trends in molecular medicine · 2017Review
- In Vitro Propagation and Branching Morphogenesis from Single Ureteric Bud Cells.Stem cell reports · 2017Article
- Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.Nature protocols · 2017Article
- Human adipose derived stem cells regress fibrosis in a chronic renal fibrotic model induced by adenine.PloS one · 2017Article
- Generation of kidney tubular organoids from human pluripotent stem cells.Scientific reports · 2016Article
- Making new kidneys: On the road from science fiction to science fact.Current opinion in organ transplantation · 2016Review
- Sox9-Positive Progenitor Cells Play a Key Role in Renal Tubule Epithelial Regeneration in Mice.Cell reports · 2016Article
- Concise Review: Understanding the Renal Progenitor Cell Niche In Vivo to Recapitulate Nephrogenesis In Vitro.Stem cells translational medicine · 2015Review
- Nephron organoids derived from human pluripotent stem cells model kidney development and injury.Nature biotechnology · 2015Article
- Cell-based therapy for kidney disease.Korean journal of urology · 2015Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The metanephric kidney is a mesodermal organ that develops as a result of reciprocal interactions between the ureteric bud and the blastema. The generation of embryonic stem (ES) cell-derived progenitors offers potential for regenerative therapies but is often limited by development of tumor formation. Because brachyury (T) denotes mesoderm specification, a mouse ES cell line with green fluorescence protein (GFP) knocked into the functional T locus as well as lacZ in the ROSA26 locus (LacZ/T/GFP) was used in cell selection and lineage tracing. In the absence of leukemia inhibitory factor, mouse ES cells give rise to embryoid bodies that can differentiate into mesoderm. Culture conditions were optimized (4 d, 10 ng/ml Activin-A) to generate maximal numbers of renal progenitor populations identified by expression of the specific combination of renal markers cadherin-11, WT-1, Pax-2, and Wnt-4. LacZ/T/GFP+ cells were further enriched by FACS selection. Five days after injection of LacZ/T/GFP+ cells into embryonic kidney explants in organ culture, beta-galactosidase immunohistochemistry showed incorporation into blastemal cells of the nephrogenic zone. After a single injection into developing live newborn mouse kidneys, co-localization studies showed that the LacZ/T/GFP+ cells were stably integrated into proximal tubules with normal morphology and normal polarization of alkaline phosphatase and aquaporin-1 for 7 mo, without teratoma formation. It is concluded that defined differentiation of ES cells into embryoid bodies with Activin-A and selection for T expression provides a means to isolate and purify renal proximal tubular progenitor cells with the potential for safe use in regenerative therapies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.