ArticleJournal of clinical microbiology2007
Isolation and propagation of the Ap-Variant 1 strain of Anaplasma phagocytophilum in a tick cell line.
Article in Journal of clinical microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed, 76 citations in OpenAlex.
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- Diversity ofAnimals : an open access journal from MDPI · 2024Article
- Aptamer selection against cell extracts containing the zoonotic obligate intracellular bacterium, Anaplasma phagocytophilum.Scientific reports · 2024Article
- Molecular Detection ofPathogens (Basel, Switzerland) · 2023Article
- Clinically differential diagnosis of human granulocytic anaplasmosis and severe fever with thrombocytopenia syndrome.Scientific reports · 2023Article
- Under-diagnosis of vector-borne diseases among individuals suspected of having Scrub Typhus in South Korea.PloS one · 2023Article
- Molecular Detection ofMicroorganisms · 2022Article
- Comparative Whole Genome Analysis of anPathogens (Basel, Switzerland) · 2022Article
- Prevalence and Genotyping ofAnimals : an open access journal from MDPI · 2022Article
- Epidemiology, Diagnosis, and Control of Canine Infectious Cyclic Thrombocytopenia and Granulocytic Anaplasmosis: Emerging Diseases of Veterinary and Public Health Significance.Veterinary sciences · 2021Review
- Case Report: The First Borrelia yangtzensis Infection in a Human in Korea.The American journal of tropical medicine and hygiene · 2021Article
- The Novel Zoonotic Pathogen,Pathogens (Basel, Switzerland) · 2021Article
- A new genetic approach to distinguish strains of Anaplasma phagocytophilum that appear not to cause human disease.Ticks and tick-borne diseases · 2021Article
- Article
- First identification of Anaplasma phagocytophilum in both a biting tick Ixodes nipponensis and a patient in Korea: a case report.BMC infectious diseases · 2020Article
- Isolation of Candidatus Rickettsia vini from Belgian Ixodes arboricola ticks and propagation in tick cell lines.Ticks and tick-borne diseases · 2020Article
- Spatio-Temporal Dynamics of Tick-Borne Diseases in North-Central Wisconsin from 2000-2016.International journal of environmental research and public health · 2020Article
- Asymptomatic-anaplasmosis confirmation using genetic and serological tests and possible coinfection with spotted fever group Rickettsia: a case report.BMC infectious diseases · 2020Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
The first tissue culture isolates of the unique Anaplasma phagocytophilum strain, Ap-Variant 1, were obtained in the Ixodes scapularis tick-derived cell line ISE6. Two isolates were from goat blood samples: one from a goat infected with I. scapularis ticks from Rhode Island and a second from a goat infected by serial passage of blood from the first infected goat. Eight isolates were made directly from I. scapularis ticks collected from white-tailed deer in Minnesota and represent the first isolations of an Anaplasma species directly from ticks. Each of the 10 isolates had a 16S rRNA gene sequence identical to that previously described for Ap-Variant 1, but differences within the ank gene were found that suggest natural variation. Prevalence of Anaplasma in the Minnesota ticks was 63.9%; 23 of 36 ticks tested by PCR were positive. Six of the tick-derived isolates were obtained from a set of 18 PCR-positive ticks, for a 33.3% isolation success rate. The conservation of host tropism among the Rhode Island and Minnesota isolates of Ap-Variant 1 was examined by use of experimental infections of mice and a goat. A Minnesota tick-derived isolate (MN-61-2) was used to inoculate naïve animals, and this isolate was able to infect a goat but unable to infect each of five mice, confirming that the Minnesota isolates have the same host tropism as Ap-Variant 1 from the northeastern United States. Light and electron microscopy of the Ap-Variant 1 isolate MN-61-2 in ISE6 cells showed cytoplasmic inclusions characteristic of A. phagocytophilum with pleomorphic bacteria in membrane-bound vacuoles and both electron-dense and electron-lucent forms.
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