ArticleJournal of virology2007
Primary cell model for activation-inducible human immunodeficiency virus.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 32 citations in OpenAlex.
- The HIV-1 reservoir in eight patients on long-term suppressive antiretroviral therapy is stable with few genetic changes over time.Proceedings of the National Academy of Sciences of the United States of America · 2013Trial
- Article
- So Pathogenic or So What?-A Brief Overview of SIV Pathogenesis with an Emphasis on Cure Research.Viruses · 2022Review
- Infection with a newly designed dual fluorescent reporter HIV-1 effectively identifies latently infected CD4eLife · 2021Article
- Article
- Finding a cure for human immunodeficiency virus-1 infection.Infectious disease clinics of North America · 2014Review
- High levels of CD2 expression identify HIV-1 latently infected resting memory CD4+ T cells in virally suppressed subjects.Journal of virology · 2013Article
- Recent developments in human immunodeficiency virus-1 latency research.The Journal of general virology · 2013Review
- Cellular and molecular mechanisms involved in the establishment of HIV-1 latency.Retrovirology · 2013Review
- Human immunodeficiency virus (HIV) latency: the major hurdle in HIV eradication.Molecular medicine (Cambridge, Mass.) · 2012Review
- Towards an HIV cure: a global scientific strategy.Nature reviews. Immunology · 2012Review
- Combination of biological screening in a cellular model of viral latency and virtual screening identifies novel compounds that reactivate HIV-1.Journal of virology · 2012Article
- A flexible model of HIV-1 latency permitting evaluation of many primary CD4 T-cell reservoirs.PloS one · 2012Article
- HIV latency in the humanized BLT mouse.Journal of virology · 2012Article
- Directly infected resting CD4+T cells can produce HIV Gag without spreading infection in a model of HIV latency.PLoS pathogens · 2012Article
- HIV latency is influenced by regions of the viral genome outside of the long terminal repeats and regulatory genes.Virology · 2011Article
- Single mutations in HIV integrase confer high-level resistance to raltegravir in primary human macrophages.Antimicrobial agents and chemotherapy · 2011Article
- HIV reservoirs and latency models.Virology · 2011Review
- The molecular biology of HIV latency: breaking and restoring the Tat-dependent transcriptional circuit.Current opinion in HIV and AIDS · 2011Review
- Establishment of HIV latency in primary CD4+ cells is due to epigenetic transcriptional silencing and P-TEFb restriction.Journal of virology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Quiescent T lymphocytes containing latent human immunodeficiency virus (HIV) provide a long-lived viral reservoir. This reservoir may be the source of active infection that is reinitiated following the cessation of antiretroviral therapy. Therefore, it is important to understand the mechanisms involved in latent infection to develop new strategies to eliminate the latent HIV reservoir. We have previously demonstrated that latently infected quiescent lymphocytes can be generated during thymopoiesis in vivo in the SCID-hu mouse system. However, there is still a pressing need for an in vitro model of HIV latency in primary human cells. Here, we present a novel in vitro model that recapitulates key aspects of dormant HIV infection. Using an enhanced green fluorescent protein-luciferase fusion protein-containing reporter virus, we have generated a stable infection in primary human CD4(+) CD8(+) thymocytes in the absence of viral gene expression. T-cell activation induces a >200-fold induction of reporter activity. The induced reporter activity originates from a fully reverse-transcribed and integrated genome. We further demonstrate that this model can be useful to study long terminal repeat regulation, as previously characterized NF-kappaB response element mutations decrease the activation of viral gene expression. This model can therefore be used to study intricate molecular aspects of activation-inducible HIV infection in primary cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.