ArticleBMC neuroscience2007
Requirement of aggregation propensity of Alzheimer amyloid peptides for neuronal cell surface binding.
Article in BMC neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 35 citations in OpenAlex.
- An effector-reduced anti-β-amyloid (Aβ) antibody with unique aβ binding properties promotes neuroprotection and glial engulfment of Aβ.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Trial
- Prodromal Alzheimer's Disease: Constitutive Upregulation of Neuroglobin Prevents the Initiation of Alzheimer's Pathology.Frontiers in neuroscience · 2020Article
- Targeting heparin and heparan sulfate protein interactions.Organic & biomolecular chemistry · 2017Review
- Fluorescence correlation spectroscopy reveals a cooperative unfolding of monomeric amyloid-β 42 with a low Gibbs free energy.Scientific reports · 2017Article
- Fluorescent filter-trap assay for amyloid fibril formation kinetics in complex solutions.ACS chemical neuroscience · 2015Article
- Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs).Chemical reviews · 2014Review
- Single-molecule imaging reveals aβ42:aβ40 ratio-dependent oligomer growth on neuronal processes.Biophysical journal · 2013Article
- Synergistic interactions between Alzheimer's Aβ40 and Aβ42 on the surface of primary neurons revealed by single molecule microscopy.PloS one · 2013Article
- Article
- Cell surface binding and internalization of aβ modulated by degree of aggregation.International journal of Alzheimer's disease · 2011Article
- Direct observation of single amyloid-β(1-40) oligomers on live cells: binding and growth at physiological concentrations.PloS one · 2011Article
- Two distinct conformations of Abeta aggregates on the surface of living PC12 cells.Biophysical journal · 2009Article
- Mechanism of neuronal versus endothelial cell uptake of Alzheimer's disease amyloid beta protein.PloS one · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAggregation of the amyloid peptides, Abeta40 and Abeta42, is known to be involved in the pathology of Alzheimer's disease (AD). Here we investigate the relationship between peptide aggregation and cell surface binding of three forms of Abeta (Abeta40, Abeta42, and an Abeta mutant).
resultsUsing confocal microscopy and flow cytometry with fluorescently labelled Abeta, we demonstrate a correlation between the aggregation propensity of the Alzheimer amyloid peptides and their neuronal cell surface association. We find that the highly aggregation prone Abeta42 associates with the surface of neuronal cells within one hour, while the less aggregation prone Abeta40 associates over 24 hours. We show that a double mutation in Abeta42 that reduces its aggregation propensity also reduces its association with the cell surface. Furthermore, we find that a cell line that is resistant to Abeta cytotoxicity, the non-neuronal human lymphoma cell line U937, does not bind either Abeta40 or Abeta42.
conclusionTaken together, our findings reveal that amyloid peptide aggregation propensity is an essential determinant of neuronal cell surface association. We anticipate that our approach, involving Abeta imaging in live cells, will be highly useful for evaluating the efficacy of therapeutic drugs that prevent toxic Abeta association with neuronal cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.