ArticleBMC microbiology2007
Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Comparative Structure Analysis of the Multi-Domain, Cell Envelope Proteases of Lactic Acid Bacteria.Microorganisms · 2023Article
- Potential of NFrontiers in microbiology · 2020Review
- Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis.BMC biotechnology · 2019Article
- TheFrontiers in microbiology · 2019Article
- Altering textural properties of fermented milk by using surface-engineered Lactococcus lactis.Microbial biotechnology · 2018Article
- Contribution of plasmid-encoded peptidase S8 (PrtP) to adhesion and transit in the gut of Lactococcus lactis IBB477 strain.Applied microbiology and biotechnology · 2017Article
- Biofilm Formation on Stainless Steel by Streptococcus thermophilus UC8547 in Milk Environments Is Mediated by the Proteinase PrtS.Applied and environmental microbiology · 2017Article
- Surface Proteins ofFrontiers in microbiology · 2017Review
- Cell Surface Properties ofFrontiers in microbiology · 2017Article
- Adhesion of the genome-sequenced Lactococcus lactis subsp. cremoris IBB477 strain is mediated by specific molecular determinants.Applied microbiology and biotechnology · 2016Article
- The Variable Regions of Lactobacillus rhamnosus Genomes Reveal the Dynamic Evolution of Metabolic and Host-Adaptation Repertoires.Genome biology and evolution · 2016Article
- Diffusion of solutes inside bacterial colonies immobilized in model cheese depends on their physicochemical properties: a time-lapse microscopy study.Frontiers in microbiology · 2015Article
- Living biointerfaces based on non-pathogenic bacteria to direct cell differentiation.Scientific reports · 2014Article
- Surface proteome analysis of a natural isolate of Lactococcus lactis reveals the presence of pili able to bind human intestinal epithelial cells.Molecular & cellular proteomics : MCP · 2013Article
- Impact of Lactococcus lactis spp. lactis Bio Adhesion on Pathogenic Bacillus cereus Biofilm on Silicone Flowing System.Indian journal of microbiology · 2013Article
- Impact of food-related environmental factors on the adherence and biofilm formation of natural Staphylococcus aureus isolates.Current microbiology · 2013Article
- Pilus biogenesis in Lactococcus lactis: molecular characterization and role in aggregation and biofilm formation.PloS one · 2012Article
- Growth phase-associated changes in the proteome and transcriptome of Lactobacillus rhamnosus GG in industrial-type whey medium.Microbial biotechnology · 2011Article
- Stochasticity of bacterial attachment and its predictability by the extended derjaguin-landau-verwey-overbeek theory.Applied and environmental microbiology · 2011Article
- Diffusion of nanoparticles in biofilms is altered by bacterial cell wall hydrophobicity.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe first step in biofilm formation is bacterial attachment to solid surfaces, which is dependent on the cell surface physico-chemical properties. Cell wall anchored proteins (CWAP) are among the known adhesins that confer the adhesive properties to pathogenic Gram-positive bacteria. To investigate the role of CWAP of non-pathogen Gram-positive bacteria in the initial steps of biofilm formation, we evaluated the physico-chemical properties and adhesion to solid surfaces of Lactococcus lactis. To be able to grow in milk this dairy bacterium expresses a cell wall anchored proteinase PrtP for breakdown of milk caseins.
resultsThe influence of the anchored cell wall proteinase PrtP on microbial surface physico-chemical properties, and consequently on adhesion, was evaluated using lactococci carrying different alleles of prtP. The presence of cell wall anchored proteinase on the surface of lactococcal cells resulted in an increased affinity to solvents with different physico-chemical properties (apolar and Lewis acid-base solvents). These properties were observed regardless of whether the PrtP variant was biologically active or not, and were not observed in strains without PrtP. Anchored PrtP displayed a significant increase in cell adhesion to solid glass and tetrafluoroethylene surfaces.
conclusionObtained results indicate that exposure of an anchored cell wall proteinase PrtP, and not its proteolytic activity, is responsible for greater cell hydrophobicity and adhesion. The increased bacterial affinity to polar and apolar solvents indicated that exposure of PrtP on lactococcal cell surface could enhance the capacity to exchange attractive van der Waals interactions, and consequently increase their adhesion to different types of solid surfaces and solvents.
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