ArticleMolecular reproduction and development2008
Expression of X-linked genes in deceased neonates and surviving cloned female piglets.
Article in Molecular reproduction and development, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Epigenetic manipulation to improve mouse SCNT embryonic development.Frontiers in genetics · 2022Review
- Advance in the Role of Epigenetic Reprogramming in Somatic Cell Nuclear Transfer-Mediated Embryonic Development.Stem cells international · 2021Review
- Locus-specific analysis of DNA methylation patterns in cloned and in vitro fertilized porcine embryos.The Journal of reproduction and development · 2020Article
- XIST Derepression in Active X Chromosome Hinders Pig Somatic Cell Nuclear Transfer.Stem cell reports · 2018Article
- Establishment of a strain of haemophilia-A pigs by xenografting of foetal testicular tissue from neonatally moribund cloned pigs.Scientific reports · 2017Article
- Factors and molecules that could impact cell differentiation in the embryo generated by nuclear transfer.Organogenesis · 2017Review
- Effects of RNAi-mediated knockdown of Xist on the developmental efficiency of cloned male porcine embryos.The Journal of reproduction and development · 2016Article
- Oxamflatin treatment enhances cloned porcine embryo development and nuclear reprogramming.Cellular reprogramming · 2015Article
- Aberrant expression of Xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos.International journal of molecular sciences · 2014Article
- Systems genetics implicates cytoskeletal genes in oocyte control of cloned embryo quality.Genetics · 2013Article
- Recent advancements in cloning by somatic cell nuclear transfer.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- X-linked gene transcription patterns in female and male in vivo, in vitro and cloned porcine individual blastocysts.PloS one · 2012Article
- RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Somatic cell nuclear transfer efficiency: how can it be improved through nuclear remodeling and reprogramming?Molecular reproduction and development · 2010Article
- Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic cell nuclear transfer porcine embryos.Cellular reprogramming · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Animal cloning through somatic cell nuclear transfer (NT) is very inefficient, probably due to insufficient reprogramming of the donor nuclei, which in turn would cause the dysregulation of gene expression. X-Chromosome inactivation (XCI) is a multi-step epigenetic process utilized by mammals to achieve dosage compensation in females. Our aim was to determine if any dysregulation of X-linked genes, which would be indicative of unfaithful reprogramming of donor nuclei, was present in cloned pigs. Real time reverse transcription polymerase chain reaction (RT-PCR) was performed to quantify the transcript levels of five X-linked genes, X inactivation-specific transcript (XIST), TSIX (the reverse spelling of XIST), hypoxanthine guanine phosphoribosyltransferase 1 (HPRT1), glucose-6-phosphate dehydrogenase (G6PD), V-raf murine sarcoma 3,611 viral oncogene homolog 1 (ARAF1), and one autosomal gene, alpha-1 type IV collagen (COL4A1) in major organs of neonatal deceased and surviving female cloned pigs as well as their age-matched control pigs from conventional breeding. Aberrant expression level of these genes was prevalent in the neonatal deceased clones, while it was only moderate in cloned pigs that survived after birth. These results suggest a correlation between the viability of the clones and the normality of their gene expression and provide a possible explanation for the death of a large portion of cloned animals around birth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.