ArticleProceedings of the National Academy of Sciences of the United States of America2007
Three-dimensional structure of cofilin bound to monomeric actin derived by structural mass spectrometry data.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Discovery of actinators, actin-derived bioactive peptides that modulate cytoskeleton and actin-related cellular activities.Science advances · 2026Article
- The multifaceted roles of aldolase A in cancer: glycolysis, cytoskeleton, translation and beyond.Human cell · 2025Review
- Sampling and Scoring in Protein-Protein Docking.Methods in molecular biology (Clifton, N.J.) · 2024Article
- RNA-based cooperative protein labeling that permits direct monitoring of the intracellular concentration change of an endogenous protein.Nucleic acids research · 2021Article
- In silico study of some selective phytochemicals against a hypothetical SARS-CoV-2 spike RBD using molecular docking tools.Computers in biology and medicine · 2021Article
- Targeting a moonlighting function of aldolase induces apoptosis in cancer cells.Cell death & disease · 2019Article
- The ClusPro web server for protein-protein docking.Nature protocols · 2017Article
- Synchrotron X-ray footprinting as a method to visualize water in proteins.Journal of synchrotron radiation · 2016Article
- Development of a microsecond X-ray protein footprinting facility at the Advanced Light Source.Journal of synchrotron radiation · 2014Article
- Synchrotron X-ray footprinting on tour.Journal of synchrotron radiation · 2014Article
- Allele-specific effects of thoracic aortic aneurysm and dissection alpha-smooth muscle actin mutations on actin function.The Journal of biological chemistry · 2011Article
- Structural NMR of protein oligomers using hybrid methods.Journal of structural biology · 2011Review
- ADF/Cofilin-actin rods in neurodegenerative diseases.Current Alzheimer research · 2010Review
- Visualizing water molecules in transmembrane proteins using radiolytic labeling methods.Biochemistry · 2010Review
- Integrated algorithms for high-throughput examination of covalently labeled biomolecules by structural mass spectrometry.Analytical chemistry · 2009Article
- Fragmentation mechanisms of oxidized peptides elucidated by SID, RRKM modeling, and molecular dynamics.Journal of the American Society for Mass Spectrometry · 2009Article
- Cofilin dissociates Arp2/3 complex and branches from actin filaments.Current biology : CB · 2009Article
- The effects of ADF/cofilin and profilin on the conformation of the ATP-binding cleft of monomeric actin.Biophysical journal · 2009Article
- Outcome of a workshop on applications of protein models in biomedical research.Structure (London, England : 1993) · 2009Article
- Role of intermonomer ionic bridges in the stabilization of the actin filament.The Journal of biological chemistry · 2008Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The cytoskeletal protein, actin, has its structure and function regulated by cofilin. In the absence of an atomic resolution structure for the actin/cofilin complex, the mechanism of cofilin regulation is poorly understood. Theoretical studies based on the similarities of cofilin and gelsolin segment 1 proposed the cleft between subdomains 1 and 3 in actin as the cofilin binding site. We used radiolytic protein footprinting with mass spectrometry and molecular modeling to provide an atomic model of how cofilin binds to monomeric actin. Footprinting data suggest that cofilin binds to the cleft between subdomains 1 and 2 in actin and that cofilin induces further closure of the actin nucleotide cleft. Site-specific fluorescence data confirm these results. The model identifies key ionic and hydrophobic interactions at the binding interface, including hydrogen-bonding between His-87 of actin to Ser-89 of cofilin that may control the charge dependence of cofilin binding. This model and its implications fill an especially important niche in the actin field, owing to the fact that ongoing crystallization efforts of the actin/cofilin complex have so far failed. This 3D binary complex structure is derived from a combination of solution footprinting data and computational approaches and outlines a general method for determining the structure of such complexes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.