ArticleGenes & development2007
A complex system of small RNAs in the unicellular green alga Chlamydomonas reinhardtii.
Article in Genes & development, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 184 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
184 citing papers in PubMed, 401 citations in OpenAlex.
- Global identification of AGO3-RNA interactions reveals targets of small RNA-mediated gene regulation in Chlamydomonas reinhardtii.Plant & cell physiology · 2025Article
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- Identification ofFrontiers in microbiology · 2025Article
- Evolution of microRNAs in Amoebozoa and implications for the origin of multicellularity.Nucleic acids research · 2024Article
- Small RNAs >26 nt in length associate with AGO1 and are upregulated by nutrient deprivation in the alga Chlamydomonas.The Plant cell · 2023Article
- Small RNA-mediated silencing of phototropin suppresses the induction of photoprotection in the green algaProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- A comparative study of microRNAs in different stages ofFrontiers in veterinary science · 2022Article
- Evolutionary conservation of nested MIR159 structural microRNA genes and their promoter characterization inFrontiers in plant science · 2022Article
- Article
- MicroRNA Expression Profile Analysis ofBioengineering (Basel, Switzerland) · 2021Article
- miR-410-3P inhibits adipocyte differentiation by targeting IRS-1 in cancer-associated cachexia patients.Lipids in health and disease · 2021Article
- Review
- Orchestral manoeuvres in the light: crosstalk needed for regulation of the Chlamydomonas carbon concentration mechanism.Journal of experimental botany · 2021Review
- The roles of microRNAs in mouse development.Nature reviews. Genetics · 2021Review
- Identification of virulence associated milRNAs and their bidirectional targets in Rhizoctonia solani and maize during infection.BMC plant biology · 2021Article
- Genome-wide identification of Argonautes in Solanaceae with emphasis on potato.Scientific reports · 2020Article
- High-throughput deep sequencing reveals the important role that microRNAs play in the salt response in sweet potato (Ipomoea batatas L.).BMC genomics · 2020Article
- The small RNA locus map for Chlamydomonas reinhardtii.PloS one · 2020Article
- Volvox and volvocine green algae.EvoDevo · 2020Review
124 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endogenous small RNAs function in RNA interference (RNAi) pathways to control gene expression through mRNA cleavage, translational repression, or chromatin modification. Plants and animals contain many microRNAs (miRNAs) that play vital roles in development, including helping to specify cell type and tissue identity. To date, no miRNAs have been reported in unicellular organisms. Here we show that Chlamydomonas reinhardtii, a unicellular green alga, encodes many miRNAs. We also show that a Chlamydomonas miRNA can direct the cleavage of its target mRNA in vivo and in vitro. We further show that the expression of some miRNAs/Candidates increases or decreases during Chlamydomonas gametogenesis. In addition to miRNAs, Chlamydomonas harbors other types of small RNAs including phased small interfering RNAs (siRNAs) that are reminiscent of plant trans-acting siRNAs, as well as siRNAs originating from protein-coding genes and transposons. Our findings suggest that the miRNA pathway and some siRNA pathways are ancient mechanisms of gene regulation that evolved prior to the emergence of multicellularity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.