ArticleApplied and environmental microbiology2007
Hexadecane and Tween 80 stimulate lipase production in Burkholderia glumae by different mechanisms.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 98 citations in OpenAlex.
- Screening of Marine Bacteria for Lipase Activity and Application as Whole-Cell Biocatalysts.Microorganisms · 2026Article
- Effects of Cocamidopropyl Betaine on In Vitro Rumen Fermentation and Enzyme Spatial Distribution, and In Vivo Digestibility and Growth Performance of Growing Yaks.Animals : an open access journal from MDPI · 2026Article
- Production of lipase and extracellular polymeric substances by the lipid-degrading bacterium Burkholderia arboris strain JYK2 in response to different substrates.World journal of microbiology & biotechnology · 2025Article
- Efficient secretory expression, purification, and characterization of lipase in Pseudomonas aeruginosa M18, with multifunctional applications in diagnostics.World journal of microbiology & biotechnology · 2025Article
- Characterization of a GDS(L)-like hydrolase from Pleurotus sapidus with an unusual SGNH motif.AMB Express · 2024Article
- Exoproteome analysis of Pseudomonas aeruginosa response to high alkane stress.Archives of microbiology · 2024Article
- Polysorbate 21 Can Modulate the Antibacterial Potential of Two Pyrazol Derivatives.Biomolecules · 2022Article
- Generation of Distinct Differentially Culturable Forms ofMicrobiology spectrum · 2022Article
- Production, purification and biochemical characterisation of a novel lipase from a newly identified lipolytic bacteriumJournal of enzyme inhibition and medicinal chemistry · 2021Article
- Strategies for Improvement of Lipid Production by YeastJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5.Applied and environmental microbiology · 2016Article
- Mutations improving production and secretion of extracellular lipase by Burkholderia glumae PG1.Applied microbiology and biotechnology · 2016Article
- The roles of the shikimate pathway genes, aroA and aroB, in virulence, growth and UV tolerance of Burkholderia glumae strain 411gr-6.Molecular plant pathology · 2014Article
- Optimization of extracellular thermophilic highly alkaline lipase from thermophilic bacillus sp isolated from hotspring of Arunachal Pradesh, India.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2012Article
- Burkholderia glumae: next major pathogen of rice?Molecular plant pathology · 2011Review
- Concomitant production of protease and lipase by Bacillus Licheniformis VSG1: Production, purification and characterization.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Burkholderia glumae strain PG1 produces a lipase of biotechnological relevance. Lipase production by this strain and its derivative LU8093, which was obtained through classical strain improvement, was investigated under different conditions. When 10% hexadecane was included in the growth medium, lipolytic activity in both strains could be increased approximately 7-fold after 24 h of growth. Hexadecane also stimulated lipase production in a strain containing the lipase gene fused to the tac promoter, indicating that hexadecane did not affect lipase gene expression at the transcriptional level, which was confirmed using lipA-gfp reporter constructs. Instead, hexadecane appeared to enhance lipase secretion, since the amounts of lipase in the culture supernatant increased in the presence of hexadecane, with a concomitant decrease in the cells, even when protein synthesis was inhibited with chloramphenicol. In the presence of olive oil as a carbon source, nonionic detergents, such as Tween 80, increased extracellular lipase activity twofold. Like hexadecane, Tween 80 appeared to stimulate lipase secretion, although in a more disruptive manner, since other, normally nonsecreted proteins were found in the culture supernatant. Additionally, like olive oil, Tween 80 was found to induce lipase gene expression in strain PG1 in medium containing sucrose as a carbon source but not in glucose-containing medium, suggesting that lipase gene expression is prone to catabolite repression. In contrast, lipase production in the lipase-overproducing strain LU8093 was independent of the presence of an inducer and was not inhibited by glucose. In conclusion, hexadecane and Tween 80 enhance lipase production in B. glumae, and they act via different mechanisms.
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