ArticleJournal of bacteriology2007
YbxF, a protein associated with exponential-phase ribosomes in Bacillus subtilis.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Article
- Ribosome induces transdifferentiation of A549 and H-111-TC cancer cell lines.Biochemistry and biophysics reports · 2021Article
- Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency.Scientific reports · 2018Article
- Article
- Polar Fixation of Plasmids during Recombinant Protein Production in Bacillus megaterium Results in Population Heterogeneity.Applied and environmental microbiology · 2015Article
- The molecular recognition of kink-turn structure by the L7Ae class of proteins.RNA (New York, N.Y.) · 2013Article
- YbxF and YlxQ are bacterial homologs of L7Ae and bind K-turns but not K-loops.RNA (New York, N.Y.) · 2012Article
- Single-molecule investigations of the stringent response machinery in living bacterial cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The origin of a derived superkingdom: how a gram-positive bacterium crossed the desert to become an archaeon.Biology direct · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ybxF gene is a member of the streptomycin operon in a wide range of gram-positive bacteria. In Bacillus subtilis, it codes for a small basic protein (82 amino acids, pI 9.51) of unknown function. We demonstrate that, in B. subtilis, YbxF localizes to the ribosome, primarily to the 50S subunit, with dependence on growth phase. Based on three-dimensional structures of YbxF generated by homology modeling, we identified helix 2 as important for the interaction with the ribosome. Subsequent mutational analysis of helix 2 revealed Lys24 as crucial for the interaction. Neither the B. subtilis ybxF gene nor its paralogue, the ymxC gene, is essential, as shown by probing DeltaybxF, DeltaymxC, or DeltaybxF DeltaymxC double deletion strains in several functional assays.
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Registered trials
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