ArticlePlant physiology2007
Functional analysis of PDX2 from Arabidopsis, a glutaminase involved in vitamin B6 biosynthesis.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 81 citations in OpenAlex.
- Multifaceted role of the vitamin B6 pathway in cancer: metabolism, immune interaction, and temporal and spatial regulation.Genes & development · 2025Review
- Functional and Bioinformatic Analysis ofGenes · 2025Article
- Pyridoxine dehydrogenase SePdx regulates photosynthesis via an association with the phycobilisome in a cyanobacterium.The Plant journal : for cell and molecular biology · 2025Article
- Piperideine-6-carboxylic acid regulates vitamin B6 homeostasis and modulates systemic immunity in plants.Nature plants · 2025Article
- Genome-wide identification of PDX and expression analysis under waterlogging stress exhibit stronger waterlogging tolerance in transgenicFrontiers in plant science · 2025Article
- PYRIDOX(AM)INE 5'-PHOSPHATE OXIDASE3 of Arabidopsis thaliana maintains carbon/nitrogen balance in distinct environmental conditions.Plant physiology · 2023Article
- Tissue-Specific Transcriptome and Metabolome Analysis Reveals the Response Mechanism ofInternational journal of molecular sciences · 2023Article
- L-Glutamine-, peptidyl- and protein-glutaminases: structural features and applications in the food industry.World journal of microbiology & biotechnology · 2022Review
- Chromosome-level genome assemblies of four wild peach species provide insights into genome evolution and genetic basis of stress resistance.BMC biology · 2022Article
- Transcriptomic Responses ofFrontiers in fungal biology · 2022Article
- Comparative proteome analyses of rhizomania resistant transgenic sugar beets based on RNA silencing mechanism.GM crops & food · 2021Article
- Crosstalk of DNA Methylation Triggered by Pathogen in Poplars With Different Resistances.Frontiers in microbiology · 2021Article
- Transcriptome and Phytochemical Analysis Reveals the Alteration of Plant Hormones, Characteristic Metabolites, and Related Gene Expression in Tea (Plants (Basel, Switzerland) · 2020Article
- Vitamin B6 Is Under a Tight Balance During Disease Development byFrontiers in plant science · 2020Article
- Clarification of the dispensability of PDX1.2 for Arabidopsis viability using CRISPR/Cas9.BMC plant biology · 2019Article
- Enhancement of vitamin BThe Plant journal : for cell and molecular biology · 2019Article
- Bacillus subtilis CBR05 induces Vitamin B6 biosynthesis in tomato through the de novo pathway in contributing disease resistance against Xanthomonas campestris pv. vesicatoria.Scientific reports · 2019Article
- Inhibition of Aminotransferases by Aminoethoxyvinylglycine Triggers a Nitrogen Limitation Condition and Deregulation of Histidine Homeostasis That Impact Root and Shoot Development and Nitrate Uptake.Frontiers in plant science · 2019Article
- Review
- Toward Eradication of B-Vitamin Deficiencies: Considerations for Crop Biofortification.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitamin B6 is an essential metabolite in all organisms, being required as a cofactor for a wide variety of biochemical reactions. De novo biosynthesis of the vitamin occurs in microorganisms and plants, but animals must obtain it from their diet. Two distinct and mutually exclusive de novo pathways have been identified to date, namely deoxyxylulose 5-phosphate dependent, which is restricted to a subset of eubacteria, and deoxyxylulose 5-phosphate independent, present in archaea, fungi, plants, protista, and most eubacteria. In these organisms, pyridoxal 5'-phosphate (PLP) formation is catalyzed by a single glutamine amidotransferase (PLP synthase) composed of a glutaminase domain, PDX2, and a synthase domain, PDX1. Despite plants being an important source of vitamin B6, very little is known about its biosynthesis. Here, we provide information for Arabidopsis thaliana. The functionality of PDX2 is demonstrated, using both in vitro and in vivo analyses. The expression pattern of PDX2 is assessed at both the RNA and protein level, providing insight into the spatial and temporal pattern of vitamin B6 biosynthesis. We then provide a detailed biochemical analysis of the plant PLP synthase complex. While the active sites of PDX1 and PDX2 are remote from each other, coordination of catalysis is much more pronounced with the plant proteins than its bacterial counterpart, Bacillus subtilis. Based on a model of the PDX1/PDX2 complex, mutation of a single residue uncouples enzyme coordination and in turn provides tangible evidence for the existence of the recently proposed ammonia tunnel through the core of PDX1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.