ArticleGenome biology2007
Functional dissection of the ash2 and ash1 transcriptomes provides insights into the transcriptional basis of wing phenotypes and reveals conserved protein interactions.
Article in Genome biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 36 citations in OpenAlex.
- Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture model.JOR spine · 2021Article
- The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival.Chromosoma · 2021Article
- Polycomb and Trithorax Group Genes inGenetics · 2017Review
- Active transcription without histone modifications.Oncotarget · 2015Article
- Absence of canonical marks of active chromatin in developmentally regulated genes.Nature genetics · 2015Article
- Microarray analysis of genes and gene functions in disc degeneration.Experimental and therapeutic medicine · 2014Article
- The BET protein FSH functionally interacts with ASH1 to orchestrate global gene activity in Drosophila.Genome biology · 2013Article
- Ash2 acts as an ecdysone receptor coactivator by stabilizing the histone methyltransferase Trr.Molecular biology of the cell · 2013Article
- dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes.Nucleic acids research · 2012Article
- Article
- dSet1 is the main H3K4 di- and tri-methyltransferase throughout Drosophila development.Genetics · 2012Article
- Widespread generation of alternative UTRs contributes to sex-specific RNA binding by UNR.RNA (New York, N.Y.) · 2012Article
- New partners in regulation of gene expression: the enhancer of Trithorax and Polycomb Corto interacts with methylated ribosomal protein l12 via its chromodomain.PLoS genetics · 2012Article
- Article
- Genome-wide profiling of diel and circadian gene expression in the malaria vector Anopheles gambiae.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing.Nucleic acids research · 2011Article
- Caenorhabditis elegans chromatin-associated proteins SET-2 and ASH-2 are differentially required for histone H3 Lys 4 methylation in embryos and adult germ cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Drosophila Myc interacts with host cell factor (dHCF) to activate transcription and control growth.The Journal of biological chemistry · 2010Article
- Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs.BMC developmental biology · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe trithorax group (trxG) genes absent, small or homeotic discs 1 (ash1) and 2 (ash2) were isolated in a screen for mutants with abnormal imaginal discs. Mutations in either gene cause homeotic transformations but Hox genes are not their only targets. Although analysis of double mutants revealed that ash2 and ash1 mutations enhance each other's phenotypes, suggesting they are functionally related, it was shown that these proteins are subunits of distinct complexes.
resultsThe analysis of wing imaginal disc transcriptomes from ash2 and ash1 mutants showed that they are highly similar. Functional annotation of regulated genes using Gene Ontology allowed identification of severely affected groups of genes that could be correlated to the wing phenotypes observed. Comparison of the differentially expressed genes with those from other genome-wide analyses revealed similarities between ASH2 and Sin3A, suggesting a putative functional relationship. Coimmunoprecipitation studies and immunolocalization on polytene chromosomes demonstrated that ASH2 and Sin3A interact with HCF (host-cell factor). The results of nucleosome western blots and clonal analysis indicated that ASH2 is necessary for trimethylation of the Lys4 on histone 3 (H3K4).
conclusionThe similarity between the transcriptomes of ash2 and ash1 mutants supports a model in which the two genes act together to maintain stable states of transcription. Like in humans, both ASH2 and Sin3A bind HCF. Finally, the reduction of H3K4 trimethylation in ash2 mutants is the first evidence in Drosophila regarding the molecular function of this trxG gene.
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