ArticleMicrobiology (Reading, England)2007
The outer membrane secretin PilQ from Neisseria meningitidis binds DNA.
Article in Microbiology (Reading, England), 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- A global perspective on the genomics ofMicrobial genomics · 2025Article
- Naturally competent bacteria and their genetic parasites-a battle for control over horizontal gene transfer?FEMS microbiology reviews · 2025Review
- Bactericidal Antibody Responses to Meningococcal Recombinant Outer Membrane Proteins.Journal of microbiology and biotechnology · 2024Article
- Basic Characterization of Natural Transformation in Avibacterium paragallinarum.Microbiology spectrum · 2023Article
- Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake inBiomolecules · 2023Article
- Article
- Pull in and Push Out: Mechanisms of Horizontal Gene Transfer in Bacteria.Frontiers in microbiology · 2018Article
- DprA from Neisseria meningitidis: properties and role in natural competence for transformation.Microbiology (Reading, England) · 2017Article
- Comparative Analyses of Transport Proteins Encoded within the Genomes of Bdellovibrio bacteriovorus HD100 and Bdellovibrio exovorus JSS.Journal of molecular microbiology and biotechnology · 2017Article
- Complete genome sequence ofStandards in genomic sciences · 2017Article
- Kinetics of DNA uptake during transformation provide evidence for a translocation ratchet mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Single-Stranded DNA Uptake during Gonococcal Transformation.Journal of bacteriology · 2016Article
- Mobile DNA in the pathogenicMicrobiology spectrum · 2015Article
- The Inner Membrane Protein PilG Interacts with DNA and the Secretin PilQ in Transformation.PloS one · 2015Article
- Concerted spatio-temporal dynamics of imported DNA and ComE DNA uptake protein during gonococcal transformation.PLoS pathogens · 2014Article
- Type-IV Pilus deformation can explain retraction behavior.PloS one · 2014Article
- DNA uptake by the nosocomial pathogen Acinetobacter baumannii occurs during movement along wet surfaces.Journal of bacteriology · 2013Article
- How hyperthermophiles adapt to change their lives: DNA exchange in extreme conditions.Extremophiles : life under extreme conditions · 2013Review
- Specific DNA recognition mediated by a type IV pilin.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Type IV pilin proteins: versatile molecular modules.Microbiology and molecular biology reviews : MMBR · 2012Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Neisseria meningitidis is naturally competent for transformation throughout its growth cycle. Transformation in neisserial species is coupled to the expression of type IV pili, which are present on the cell surface as bundled filamentous appendages, and are assembled, extruded and retracted by the pilus biogenesis components. During the initial phase of the transformation process, binding and uptake of DNA takes place with entry through a presumed outer-membrane channel into the periplasm. This study showed that DNA associates only weakly with purified pili, but binds significantly to the PilQ complex isolated directly from meningococcal membranes. By assessing the DNA-binding activity of the native complex PilQ, as well as recombinant truncated PilQ monomers, it was shown that the N-terminal region of PilQ is involved in the interaction with DNA. It was evident that the binding of ssDNA to PilQ had a higher affinity than the binding of dsDNA. The binding of DNA to PilQ did not, however, depend on the presence of the neisserial DNA-uptake sequence. It is suggested that transforming DNA is introduced into the cell through the outer-membrane channel formed by the PilQ complex, and that DNA uptake occurs by non-specific introduction of DNA coupled to pilus retraction, followed by presentation to DNA-binding component(s), including PilQ.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.