ArticleBioconjugate chemistry
Peptide beacons: a new design for polypeptide-based optical biosensors.
Article in Bioconjugate chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- Bio-Inspired Drug Delivery Systems: From Synthetic Polypeptide Vesicles to Outer Membrane Vesicles.Pharmaceutics · 2023Review
- Amino Acid-Viologen Hybrids: Synthesis, Cucurbituril Host-Guest Chemistry, and Implementation on the Production of Peptides.The Journal of organic chemistry · 2022Article
- A Review: Evolution and Diversity of Optical Fibre Plasmonic Sensors.Sensors (Basel, Switzerland) · 2019Review
- RNA polymerase molecular beacon as tool for studies of RNA polymerase-promoter interactions.Methods (San Diego, Calif.) · 2015Review
- Efficient Synthesis of Peptide and Protein Functionalized Pyrrole-Imidazole Polyamides Using Native Chemical Ligation.International journal of molecular sciences · 2015Article
- Photoresponsive supramolecular complexes as efficient DNA regulator.Scientific reports · 2014Article
- Design of peptide affinity ligands for S-protein: a comparison of combinatorial and de novo design strategies.Molecular diversity · 2013Article
- Molecular beacons of xeno-nucleic acid for detecting nucleic acid.Theranostics · 2013Review
- Assisted peptide folding by surface pattern recognition.Biophysical journal · 2011Article
- Interaction of Escherichia coli RNA polymerase σ70 subunit with promoter elements in the context of free σ70, RNA polymerase holoenzyme, and the β'-σ70 complex.The Journal of biological chemistry · 2011Article
- Converting a protein into a switch for biosensing and functional regulation.Protein science : a publication of the Protein Society · 2011Review
- Structure-switching biosensors: inspired by Nature.Current opinion in structural biology · 2010Review
- Folding-based electrochemical biosensors: the case for responsive nucleic acid architectures.Accounts of chemical research · 2010Article
- The length and viscosity dependence of end-to-end collision rates in single-stranded DNA.Biophysical journal · 2009Article
- Beyond molecular beacons: optical sensors based on the binding-induced folding of proteins and polypeptides.Chemistry (Weinheim an der Bergstrasse, Germany) · 2009Article
- Chimeric peptide beacons: a direct polypeptide analog of DNA molecular beacons.Chemical communications (Cambridge, England) · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Both epitope mapping and other in vitro selection techniques produce short polypeptides that tightly and specifically bind to any of a wide range of macromolecular targets. Here, we demonstrate a potentially general means of converting such polypeptides into optical biosensors. The sensing architecture we have developed, termed peptide beacons, is based on the observation that, whereas short peptides are almost invariably unfolded and highly dynamic, they become rigid when complexed to a macromolecular target. Using this effect to segregate a long-lived fluorophore from an electron transfer based, contact quencher (both covalently attached to the peptide), we have produced a robust optical sensor for anti-HIV antibodies. The binding-induced segregation of the fluorophore-quencher pair produces a 6-fold increase in sensor emission, thus allowing us to readily detect as low as approximately 250 pM of the target antibody. Because the sensor is based on binding-induced folding and a visible-light fluorophore, it is sufficiently selective to work directly in complex, contaminant-ridden samples such as saliva and blood.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.