Trial reportWorld journal of gastroenterology2007
Stool-based DNA testing, a new noninvasive method for colorectal cancer screening, the first report from Iran.
Trial report in World journal of gastroenterology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 4 syntheses or guidelines pooled it, 42 citations in OpenAlex.
- Fecal DNA methylation markers for detecting stages of colorectal cancer and its precursors: a systematic review.Clinical epigenetics · 2020Pooled it
- The importance of stool DNA methylation in colorectal cancer diagnosis: A meta-analysis.PloS one · 2018Pooled it
- The Diagnostic Performance of Stool DNA Testing for Colorectal Cancer: A Systematic Review and Meta-Analysis.Medicine · 2016Pooled it
- Accuracy of early detection of colorectal tumours by stool methylation markers: a meta-analysis.World journal of gastroenterology · 2014Pooled it
- A scoping review of DNA methylation biomarkers for non-invasive detection of colorectal cancer at the CpG site level.Clinical epigenetics · 2026Article
- Pathogenesis and biomarkers of colorectal cancer by epigenetic alteration.Intestinal research · 2024Review
- Multi-Omics Analysis Demonstrates the Critical Role of Non-Ethanolic Components of Alcoholic Beverages in the Host Microbiome and Metabolome: A Human- and Animal-Based Study.Microorganisms · 2023Article
- Ketogenic Diet and Beta-Hydroxybutyrate in Colorectal Cancer.DNA and cell biology · 2022Article
- Non-collagenous extracellular matrix protein dermatopontin may play a role as another component of transforming growth factor-β signaling pathway in colon carcinogenesis.Iranian journal of basic medical sciences · 2021Article
- [A non-invasive method for detecting mitochondrial tRNANan fang yi ke da xue xue bao = Journal of Southern Medical University · 2021Article
- Relationship betweenAnnals of medicine and surgery (2012) · 2020Article
- Comparison and evaluation of four methods for extracting DNA fromJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- DNA methylation patterns as noninvasive biomarkers and targets of epigenetic therapies in colorectal cancer.Epigenomics · 2016Review
- Epigenetic Alterations in Colorectal Cancer: Emerging Biomarkers.Gastroenterology · 2015Review
- Colorectal cancer in iran: molecular epidemiology and screening strategies.Journal of cancer epidemiology · 2015Review
- DNA methylation analysis of SFRP2, GATA4/5, NDRG4 and VIM for the detection of colorectal cancer in fecal DNA.Oncology letters · 2014Article
- p33Oncology letters · 2014Article
- Advances in epigenetic biomarker research in colorectal cancer.World journal of gastroenterology · 2014Review
- Circulating and stool nucleic acid analysis for colorectal cancer diagnosis.World journal of gastroenterology · 2014Review
- Cell-free nucleic acids as noninvasive biomarkers for colorectal cancer detection.Frontiers in genetics · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo detect tumor-associated DNA changes in stool samples among Iranian patients with colorectal cancer (CRC) compared to healthy individuals using BAT-26, p16 hypermethylation and long DNA markers.
methodsStool DNA was isolated from 45 subjects including 25 CRC patients and 20 healthy individuals using a new, fast and easy extraction method. Long DNA associated with tumor was detected using polymerase chain reaction method. Microsatellite studies were performed utilizing denaturating polyacrylamide gel to determine the instability of BAT-26. Methylation status of p16 promoter was analyzed using methylation-specific PCR (MSP).
resultsThe results showed a significant difference in existence of long DNA (16 in patients vs 1 in controls, P < 0.001) and p16 (5 in patients vs none in controls, P = 0.043) in the stool samples of two groups. Long DNA was detected in 64% of CRC patients; whereas just one of the healthy individuals was positive for Long DNA. p16 methylation was found in 20% of patients and in none of healthy individuals. Instability of BAT-26 was not detected in any of stool samples.
conclusionWe could detect colorectal cancer related genetic alterations by analyzing stool DNA with a sensitivity of 64% and 20% and a specificity of 95% and 100% for Long DNA and p16 respectively. A non-invasive molecular stool-based DNA testing can provide a screening strategy in high-risk individuals. However, additional testing on more samples is necessary from Iranian subjects to determine the exact specificity and sensitivity of these markers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.