ArticleKorean journal of ophthalmology : KJO2007
Insulin enhances nitric oxide production in trabecular meshwork cells via de novo pathway for tetrahydrobiopterin synthesis.
Article in Korean journal of ophthalmology : KJO, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- A FAERS database study on visual impairment adverse events associated with three long-acting insulin analogues.Frontiers in endocrinology · 2026Article
- Impact of Type 2 diabetes mellitus and insulin use on progression to glaucoma surgery in primary open angle glaucoma.Eye (London, England) · 2024Observational
- Is There a Role for Bioactive Lipids in the Pathobiology of Diabetes Mellitus?Frontiers in endocrinology · 2017Article
- Shear stress-triggered nitric oxide release from Schlemm's canal cells.Investigative ophthalmology & visual science · 2014Article
- Is Glaucoma a Neurodegeneration caused by Central Insulin Resistance: Diabetes Type 4?Journal of current glaucoma practiceArticle
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1 author.
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Abstract
purposeTo investigate the effect of insulin on the production of nitric oxide (NO) in the trabecular meshwork (TM) cells and the enzymatic synthetic pathway of tetrahydrobiopterin (BH(4)) synthesis.
methodsPrimarily cultured human TM cells were exposed to 1, 10, and 100 microgram/ml of insulin and 0, 1, 10, 100 and 1000 nM dexamethasone for 3 days. To evaluate the enzymatic pathway of BH(4) synthesis, 10 microM dexamethasone, 5 mM diaminopyrimidinone, 100 microM ascorbic acid, 100 microM sepiapterin, or 10 microM methotrexate were also co-administered respectively. Cellular survival and NO production were measured with MTT and Griess assay.
resultsInsulin enhanced NO production in a dose-dependent manner significantly (0.05) without affecting cell viability, whereas dexamethasone inhibited NO production. With co-exposure of insulin, diaminopyrimidinone and sepiapterin inhibited insulin-induced NO production. Ascorbic acid increased NO production independent of insulin and methotrexate did not affect to the action of insulin in NO production.
conclusionsInsulin increases NO production in TM cells via de novo synthetic pathway for BH(4) synthesis. Insulin could be involved in the regulation of trabecular outflow by enhancing NO production in TM cells.
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