ArticleProtoplasma2007
Actin-filament-dependent remodeling of the vacuole in cultured mesophyll protoplasts.
Article in Protoplasma, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Response surface methodology mediated optimization of phytosulfokine and plant growth regulators for enhanced protoplast division, callus induction, and somatic embryogenesis in Angelica Gigas Nakai.BMC plant biology · 2024Article
- Cellular dynamics of coenocytic endosperm development in Arabidopsis thaliana.Nature plants · 2023Article
- Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells.The Plant cell · 2022Article
- Taro raphide-associated proteins: Allergens and crystal growth.Plant direct · 2022Article
- Cytoskeletal organization in isolated plant cells under geometry control.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming.International journal of molecular sciences · 2020Review
- Vacuolar membrane structures and their roles in plant-pathogen interactions.Plant molecular biology · 2019Review
- The relationship between vacuolation and initiation of PCD in rice (Oryza sativa) aleurone cells.Scientific reports · 2017Article
- Interactions between plant endomembrane systems and the actin cytoskeleton.Frontiers in plant science · 2015Review
- Peroxisomes contribute to reactive oxygen species homeostasis and cell division induction in Arabidopsis protoplasts.Frontiers in plant science · 2015Article
- Live Cell Imaging During Germination Reveals Dynamic Tubular Structures Derived from Protein Storage Vacuoles of Barley Aleurone Cells.Plants (Basel, Switzerland) · 2014Article
- Plant vacuole morphology and vacuolar trafficking.Frontiers in plant science · 2014Review
- Progress in plant protoplast research.Planta · 2013Review
- Protoplasts: a useful research system for plant cell biology, especially dedifferentiation.Protoplasma · 2013Review
- Arabidopsis VILLIN2 and VILLIN3 are required for the generation of thick actin filament bundles and for directional organ growth.Plant physiology · 2012Article
- One protoplast is not the other!Plant physiology · 2011Article
- The plant-specific actin binding protein SCAB1 stabilizes actin filaments and regulates stomatal movement in Arabidopsis.The Plant cell · 2011Article
- Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array.The Journal of cell biology · 2009Article
- Array and distribution of actin filaments in guard cells contribute to the determination of stomatal aperture.Plant cell reports · 2008Article
- Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells.Plant signaling & behavior · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of plant cells to dedifferentiate represents an important survival strategy invoked in a range of situations from repair mechanisms following wounding to apomixis. Dedifferentiation requires that somatic cells reprogram and enter the cell division cycle. This in turn necessitates the accurate partitioning of nuclear content and organelles, such as chloroplasts, to daughter cells, thereby ensuring continuity of cellular information systems. The distribution of cytoplasm and its organelle content in mature plant cells is governed by a large, central vacuole, with connections between distant cortical and perinuclear cytoplasmic domains mediated by transvacuolar strands. Here we examined the changes to vacuolar architecture in Arabidopsis thaliana protoplasts expressing a green-fluorescent protein fusion to a delta-tonoplast-intrinsic protein (deltaTIP). We found that vacuolar architecture became increasingly intricate during protoplast culture with the development of numerous transvacuolar strands. The development of an intricate vacuolar architecture was an actin filament- and not microtubule-dependent process, as is the case in interphase plant cells. Furthermore, we show that myosin is required for this increased complexity of vacuolar architecture and the formation of subcortical actin filament arrays. Despite the likelihood that increased vacuolar invagination would allow better redistribution of cytoplasmic organelles, we found that repositioning of chloroplasts from cortical to perinuclear cytoplasm was not dependent on transvacuolar strands. Our findings indicate that the vacuole is a dynamic entity that develops a complex architecture before dedifferentiating plant cells enter cell division.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.