ArticleApplied microbiology and biotechnology2007
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Article in Applied microbiology and biotechnology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 100 citations in OpenAlex.
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- Metabolic engineering of Bacillus subtilis toward the efficient and stable production of CAMB Express · 2023Article
- Seasonal activities of the phyllosphere microbiome of perennial crops.Nature communications · 2023Article
- Classification and Multifaceted Potential of Secondary Metabolites Produced byMolecules (Basel, Switzerland) · 2023Review
- Microbial (Frontiers in bioengineering and biotechnology · 2022Review
- Positioning Bacillus subtilis as terpenoid cell factory.Journal of applied microbiology · 2021Review
- Are Bacterial Persisters Dormant Cells Only?Frontiers in microbiology · 2021Review
- Advances in Enhanced Menaquinone-7 Production FromFrontiers in bioengineering and biotechnology · 2021Review
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- Production of Squalene inJournal of agricultural and food chemistry · 2020Article
- From Modules to Networks: a Systems-Level Analysis of the Bacitracin Stress Response in Bacillus subtilis.mSystems · 2020Article
- Draft Genome Sequence ofMicrobiology resource announcements · 2020Article
- Impact of Fatty-Acid Labeling ofFrontiers in microbiology · 2020Article
- Overview of the Antimicrobial Compounds Produced by Members of theFrontiers in microbiology · 2019Review
- Metabolic Engineering ofFrontiers in microbiology · 2019Article
- Metabolic engineering for the production of isoprene and isopentenol by Escherichia coli.Applied microbiology and biotechnology · 2018Review
- A second RNA-binding protein is essential for ethanol tolerance provided by the bacterial OLE ribonucleoprotein complex.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Large Noncoding RNAs in Bacteria.Microbiology spectrum · 2018Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In comparison to other bacteria Bacillus subtilis emits the volatile compound isoprene in high concentrations. Isoprene is the smallest representative of the natural product group of terpenoids. A search in the genome of B. subtilis resulted in a set of genes with yet unknown function, but putatively involved in the methylerythritol phosphate (MEP) pathway to isoprene. Further identification of these genes would give the possibility to engineer B. subtilis as a host cell for the production of terpenoids like the valuable plant-produced drugs artemisinin and paclitaxel. Conditional knock-out strains of putative genes were analyzed for the amount of isoprene emitted. Differences in isoprene emission were used to identify the function of the enzymes and of the corresponding selected genes in the MEP pathway. We give proof on a biochemical level that several of these selected genes from this species are involved in isoprene biosynthesis. This opens the possibilities to investigate the physiological function of isoprene emission and to increase the endogenous flux to the terpenoid precursors, isopentenyl diphosphate and dimethylallyl diphosphate, for the heterologous production of more complex terpenoids in B. subtilis.
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