Evidence map›Paper›PMID 17456783›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2007

Phosphatidylethanolamine N-methyltransferase (PEMT) gene expression is induced by estrogen in human and mouse primary hepatocytes.

Mary Resseguie, Jiannan Song, Mihai D Niculescu, Kerry-Ann da Costa, Thomas A Randall, Steven H Zeisel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
135citing papers in PubMed, 3 pooled it
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

135 citing papers in PubMed, 3 syntheses or guidelines pooled it, 238 citations in OpenAlex.

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75 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Mary ResseguieDepartment of Nutrition, School of Public Health and School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7461, USA.
Jiannan Song
Mihai D Niculescu
Kerry-Ann da Costa
Thomas A Randall
Steven H Zeisel
University of North Carolina at Chapel Hill · US

Funding

UNC-CH CENTER FOR ENVIRONMENTAL HEALTH &SUSCEPTIBILITYP30ES010126 · NIEHS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Melissa A. Troester · 2001 to 2026
$36.3M
UNIV OF NORTH CAROLINA CLINICAL NUTRITION RESEARCH UNITP30DK056350 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI SUSAN M. SMITH · 1999 to 2026
$31.6M
TIME FRAMES OF CHOLINE ENHANCEMENT OF MEMORYP01AG009525 · NIA · BOSTON UNIVERSITY MEDICAL CAMPUS · PI BLUSZTAJN, JAN KRZYSZTOF · 1991 to 2010
$11.8M
HUMAN REQUIREMENTS FOR THE NUTRIENT CHOLINER01DK055865 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ZEISEL, STEVEN H · 2000 to 2011
$7.6M
NIA NIH HHS AG09525NIA NIH HHS P01 AG009525NIDDK NIH HHS DK55865NIDDK NIH HHS DK56350NIDDK NIH HHS P30 DK056350NIDDK NIH HHS R01 DK055865NIEHS NIH HHS ES10126NIEHS NIH HHS P30 ES010126
6 · The paper itself

Abstract

Choline is an essential nutrient for humans, though some of the requirement can be met by endogenous synthesis catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). Premenopausal women are relatively resistant to choline deficiency compared with postmenopausal women and men. Studies in animals suggest that estrogen treatment can increase PEMT activity. In this study we investigated whether the PEMT gene is regulated by estrogen. PEMT transcription was increased in a dose-dependent manner when primary mouse and human hepatocytes were treated with 17-beta-estradiol for 24 h. This increased message was associated with an increase in protein expression and enzyme activity. In addition, we report a region that contains a perfect estrogen response element (ERE) approximately 7.5 kb from the transcription start site corresponding to transcript variants NM_007169 and NM-008819 of the human and murine PEMT genes, respectively, three imperfect EREs in evolutionarily conserved regions and multiple imperfect EREs in nonconserved regions in the putative promoter regions. We predict that both the mouse and human PEMT genes have three unique transcription start sites, which are indicative of either multiple promoters and/or alternative splicing. This study is the first to explore the underlying mechanism of why dietary requirements for choline vary with estrogen status in humans.

Indexed as

Alternative SplicingAnimalsCells, CulturedCholine DeficiencyEstradiolFemaleGene Expression Regulation, EnzymologicHepatocytesHumansMaleMicePhosphatidylethanolamine N-MethyltransferasePostmenopausePremenopausePromoter Regions, GeneticRNA, MessengerEstradiolPEMT protein, humanPhosphatidylethanolamine N-MethyltransferaseRNA, Messenger

Identifiers

PMID17456783
PMCPMC2430895
OpenAlexW2028294233

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.