ArticleThe American journal of pathology2007
Epigenetic reversion of breast carcinoma phenotype is accompanied by changes in DNA sequestration as measured by AluI restriction enzyme.
Article in The American journal of pathology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Tumor reversion: a dream or a reality.Biomarker research · 2021Review
- Cancer Stem Cells in Pheochromocytoma and Paraganglioma.Frontiers in endocrinology · 2020Review
- Baicalein inhibits fibronectin-induced epithelial-mesenchymal transition by decreasing activation and upregulation of calpain-2.Cell death & disease · 2019Article
- ITIH5 mediates epigenetic reprogramming of breast cancer cells.Molecular cancer · 2017Article
- Mammary epithelial cell interactions with fibronectin stimulate epithelial-mesenchymal transition.Oncogene · 2014Article
- Cancer stem cells: progress and challenges in lung cancer.Stem cell investigation · 2014Review
- Increased Resistance of Breast, Prostate, and Embryonic Carcinoma Cells against Herpes Simplex Virus in Three-Dimensional Cultures.ISRN oncology · 2013Article
- Breast cancer cells in three-dimensional culture display an enhanced radioresponse after coordinate targeting of integrin alpha5beta1 and fibronectin.Cancer research · 2010Article
- The difficulty of targeting cancer stem cell niches.Clinical cancer research : an official journal of the American Association for Cancer Research · 2010Review
- Seed in soil, with an epigenetic view.Biochimica et biophysica acta · 2009Review
- Tissue architecture and function: dynamic reciprocity via extra- and intra-cellular matrices.Cancer metastasis reviews · 2009Review
- Cancer and epigenetic reversion--the fundamental role of redox.The American journal of pathology · 2007Article
- The tumor microenvironment is a dominant force in multidrug resistance.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapyReview
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The importance of microenvironment and context in regulation of tissue-specific genes is well established. DNA exposure to or the sequestration from nucleases detects differences in higher order chromatin structure in intact cells without disturbing cellular or tissue architecture. To investigate the relationship between chromatin organization and tumor phenotype, we used an established three-dimensional assay in which normal and malignant human breast cells can be easily distinguished by the morphology of the structures they make (acinus-like versus tumor-like, respectively). We show that these phenotypes can be distinguished also by sensitivity to AluI digestion in which the malignant cells resist digestion relative to nonmalignant cells. Treatment of T4-2 breast cancer cells in three-dimensional culture with cAMP analogs or a phosphatidylinositol 3-kinase inhibitor not only reverted their phenotype from nonpolar to polar acinar-like structures but also enhanced chromatin sensitivity to AluI. By using different cAMP analogs, we show that cAMP-induced phenotypic reversion, polarization, and shift in DNA organization act through a cAMP-dependent protein-kinase A-coupled signaling pathway. Importantly, inhibitory antibody to fibronectin produced the same effect. These experiments underscore the concept that modifying the tumor microenvironment can alter the organization of tumor cells and demonstrate that architecture and global chromatin organization are coupled and highly plastic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.