ArticleFolia microbiologica2006
Development of specific and rapid detection of bacterial pathogens in dairy products by PCR.
Article in Folia microbiologica, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Virulence Factor Genes and Cytotoxicity ofMicrobiology spectrum · 2022Article
- Effect of prophylactic vitamin C administration on the efficiency of florfenicol or sulfadiazine-trimethoprim antimicrobial therapy in chickens with staphylococcal arthritis.Tropical animal health and production · 2021Article
- Characterization of Clinical and CarrierViruses · 2020Article
- Limit of detection of genomic DNA by conventional PCR for estimating the load of Staphylococcus aureus and Escherichia coli associated with bovine mastitis.Folia microbiologica · 2015Article
- Incidence of staphylococci and streptococci during winter in mastitic milk of sahiwal cow and murrah buffaloes.Indian journal of microbiology · 2012Article
- Occurrence and antimicrobial drug susceptibility patterns of commensal and diarrheagenic Escherichia coli in fecal microbiota from children with and without acute diarrhea.Journal of microbiology (Seoul, Korea) · 2011Article
- Detection of Group B Streptococcus in Brazilian pregnant women and antimicrobial susceptibility patterns.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Diarrheagenic Escherichia coli strains recovered from urban pigeons (Columba livia) in Brazil and their antimicrobial susceptibility patterns.Current microbiology · 2009Article
- N-terminal anchor in surface immunogenic protein of Streptococcus agalactiae and its influence on immunity elicitation.Folia microbiologica · 2009Article
- Postnatal development of bacterial population in the gastrointestinal tract of calves.Folia microbiologica · 2007Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple and specific method for direct detection of bovine mastitis pathogens (Streptococcus agalactiae (GBS), Staphylococcus aureus and Escherichia coli) in milk products, bacterial samples from milk and isolated bacterial DNA was developed. The method is based on polymerase chain reaction (PCR) using sequence-specific primers only for GBS and species-specific primers derived from 16S and 23S rRNA for all chosen species. The presence of the gene of surface immunogenic protein (Sip) in bovine GBS isolates, described previously only in human GBS isolates was confirmed. The GBS detection was performed with the sequence coding for surface immunogenic protein from GBS human isolates designated as Sip specific sequence (SSS); this sequence was selected for specific primer design. The sequence is unique for GBS and was designed from a consensus of all known sip genes. The specific identification was shown on a collection of 75 GBS bovine isolates from different localities in Slovakia. All isolates were positive to SSS, 16S and 23S rRNA sequence. The 16S and 23S rRNA PCR detection was also performed with S. aureus and E. coli isolates and specific PCR products were also detected. The detection limit of this assay for milk products was 6 CFU/microL (i.e. 6000 CFU/mL) for GBS and E. coli, and 16 CFU/microL for S. aureus. This rapid, sensitive and specific diagnostic method can be performed within hours and represents an innovative diagnostic tool for the detection of milk pathogens in dairy products.
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