Evidence map›Paper›PMID 17455764›Full record

ReviewScience progress2007

Current molecular techniques for the detection of microbial pathogens.

Luca Galluzzi, Mauro Magnani, Nick Saunders, Carsten Harms, Ian James Bruce

Abstract readReview
In one paragraph

Review in Science progress, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Towards deployable CRISPR-based nucleic acid detection.Progress in biomedical engineering (Bristol, England) · 2026
    Review
  3. Review
  4. Article
  5. Review
  6. Serological and Molecular Detection ofFrontiers in veterinary science · 2022
    Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. PCR identification of toxic euglenid speciesJournal of applied phycology · 2018
    Article
  12. Article
  13. Enumeration of Mycobacterium leprae using real-time PCR.PLoS neglected tropical diseases · 2008
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Luca GalluzziIstituto di Chimica Biologica G. Fornaini, Università degli Studi Carlo Bo, Via Saffi, 2, 61029 Urbino, Italy.
Mauro Magnani
Nick Saunders
Carsten Harms
Ian James Bruce

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditionally the detection of microbial pathogens in clinical, environmental or food samples has commonly needed the prelevation of cells by culture before the application ofthe detection strategy. This is done to increase cell number thereby overcoming problems associated with the sensitivity of classical detection strategies. However, culture-based methods have the disadvantages of taking longer, usually are more complex and require skilled personnel as well as not being able to detect viable but non cultivable microbial species. A number of molecular methods have been developed in the last 10 to 15 years to overcome these issues and to facilitate the rapid, accurate, sensitive and cost effective identification and enumeration of microorganisms which are designed to replace and/or support classical approaches to microbial detection. Amongst these new methods, ones based on the polymerase chain reaction and nucleic acid hybridization have been shown to be particularly suitable for this purpose. This review generally summarizes some of the current and emerging nucleic acid based molecular approaches for the detection, discrimination andquantification ofmicrobes in environmental, food and clinical samples and includes reference to the recently developing areas of microfluidics and nanotechnology "Lab-on-a-chip".

Indexed as

Microbiological TechniquesAutomationBiotechnologyCells, CulturedClinical Laboratory TechniquesMolecular BiologyNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionPolymorphism, Restriction Fragment Length

Identifiers

PMID17455764
PMCPMC10361161

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.