ArticlePlant cell reports2007
Agrobacterium-mediated transformation of the dwarf pomegranate (Punica granatum L. var. nana).
Article in Plant cell reports, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A stable transformation platform in pomegranate uncovers PgMYB10 as a key regulator of anthocyanin biosynthesis.Plant cell reports · 2026Article
- Eco-friendly Suppression of Xanthomonas axonopodis pv. punicae: the Role of Lactobacillus helveticus PMP-6 in Pomegranate Disease Management.Current microbiology · 2026Article
- An effective method for establishing a regeneration and genetic transformation system forFrontiers in plant science · 2023Article
- Fine Mapping of the "black" Peel Color in Pomegranate (Frontiers in plant science · 2021Article
- A robust genetic transformation protocol to obtain transgenic shoots ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. 'Malas Saveh' and 'Yusef Khani'.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Article
- Article
- Tissue culture-mediated biotechnological intervention in pomegranate: a review.Plant cell reports · 2011Review
- Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur.Plant cell reports · 2010Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dwarf pomegranate (Punica granatum L. var. nana) is a dwarf ornamental plant that has the potential to be the model plant of perennial fruit trees because it bears fruits within 1 year of seedling. We established an Agrobacterium-mediated transformation system for the dwarf pomegranate. Adventitious shoots regenerated from leaf segments were inoculated with A. tumefaciens strain EHA105 harboring the binary vector pBin19-sgfp, which contains neomycin phosphotransferase (npt II) and green fluorescent protein (gfp) gene as a selectable and visual marker, respectively. After co-cultivation, the inoculated adventitious shoots were cut into small pieces to induce regeneration, and then selected on MS medium supplemented with 0.5 muM alpha-naphthaleneacetic acid (NAA), 5 muM N(6)-benzyladenine (BA), 0.3% gellan gum, 50 mg/l kanamycin, and 10 mg/l meropenem. Putative transformed shoots were regenerated after 6-8 months of selection. PCR and PCR-Southern blot analysis revealed the integration of the transgene into the plant genome. Transformants bloomed and bore fruits within 3 months of being potted, and the inheritance of the transgene was confirmed in T(1) generations. The advantage of the transformation of dwarf pomegranate was shown to be the high transformation rate. The establishment of this transformation system is invaluable for investigating fruit-tree-specific phenomena.
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