ArticlePlant molecular biology2007
Improved drought tolerance without undesired side effects in transgenic plants producing trehalose.
Article in Plant molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers, 2 of them syntheses that pooled it.
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Who cites it
60 citing papers in PubMed, 2 syntheses or guidelines pooled it, 217 citations in OpenAlex.
- Identification of conserved drought-adaptive genes using a cross-species meta-analysis approach.BMC plant biology · 2015Pooled it
- Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa.BMC genomics · 2009Pooled it
- Desiccation tolerance mechanisms in the green lineage and beyond.The New phytologist · 2026Review
- Pan-genomic analysis and abiotic stress expression of eight TPS gene families in Brassica napus.BMC plant biology · 2026Article
- The role of trehalose metabolism in plant stress tolerance.Journal of advanced research · 2025Review
- Genome-Wide Identification and Expression Analysis of the Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Gene Families in Rose (Plants (Basel, Switzerland) · 2023Article
- Genome- and transcriptome-wide identification of trehalose-6-phosphate phosphatases (TPP) gene family and their expression patterns under abiotic stress and exogenous trehalose in soybean.BMC plant biology · 2023Article
- Trehalose accumulation enhances drought tolerance by modulating photosynthesis and ROS-antioxidant balance in drought sensitive and tolerant rice cultivars.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- The genetic variation in drought resistance in eighteen perennial ryegrass varieties and the underlying adaptation mechanisms.BMC plant biology · 2023Article
- MicroRNA172b-5p/trehalose-6-phosphate synthase module stimulates trehalose synthesis and microRNA172b-3p/AP2-like module accelerates flowering in barley upon drought stress.Frontiers in plant science · 2023Article
- Genome-wide identification ofFrontiers in plant science · 2023Article
- Plant hormone signals regulate trehalose accumulation against osmotic stress in watermelon cells.Protoplasma · 2022Article
- Genome-Wide Identification of Cotton (Plants (Basel, Switzerland) · 2022Article
- Article
- Functional and molecular characterization of fluoride exporter (FEX) from rice and its constitutive overexpression in Nicotiana benthamiana to promote fluoride tolerance.Plant cell reports · 2021Article
- Metabolomics and Molecular Approaches Reveal Drought Stress Tolerance in Plants.International journal of molecular sciences · 2021Review
- Understanding the roles of osmolytes for acclimatizing plants to changing environment: a review of potential mechanism.Plant signaling & behavior · 2021Review
- Metabolomic and Biochemical Analysis of Two Potato (Plants (Basel, Switzerland) · 2021Article
- Metabolic engineering of osmoprotectants to elucidate the mechanism(s) of salt stress tolerance in crop plants.Planta · 2021Review
- Trehalose-6-phosphate phosphatase E modulates ABA-controlled root growth and stomatal movement in Arabidopsis.Journal of integrative plant biology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most organisms naturally accumulating trehalose upon stress produce the sugar in a two-step process by the action of the enzymes trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Transgenic plants overexpressing TPS have shown enhanced drought tolerance in spite of minute accumulation of trehalose, amounts believed to be too small to provide a protective function. However, overproduction of TPS in plants has also been found combined with pleiotropic growth aberrations. This paper describes three successful strategies to circumvent such growth defects without loosing the improved stress tolerance. First, we introduced into tobacco a double construct carrying the genes TPS1 and TPS2 (encoding TPP) from Saccharomyces cerevisiae. Both genes are regulated by an Arabidopsis RuBisCO promoter from gene AtRbcS1A giving constitutive production of both enzymes. The second strategy involved stress-induced expression by fusing the coding region of ScTPS1 downstream of the drought-inducible Arabidopsis AtRAB18 promoter. In transgenic tobacco plants harbouring genetic constructs with either ScTPS1 alone, or with ScTPS1 and ScTPS2 combined, trehalose biosynthesis was turned on only when the plants experienced stress. The third strategy involved the use of AtRbcS1A promoter together with a transit peptide in front of the coding sequence of ScTPS1, which directed the enzyme to the chloroplasts. This paper confirms that the enhanced drought tolerance depends on unknown ameliorated water retention as the initial water status is the same in control and transgenic plants and demonstrates the influence of expression of heterologous trehalose biosynthesis genes on Arabidopsis root development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.