ArticleMolecular and cellular biology2007
Yeast Rpb9 plays an important role in ubiquitylation and degradation of Rpb1 in response to UV-induced DNA damage.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Elf1 promotes Rad26's interaction with lesion-arrested Pol II for transcription-coupled repair.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- RNAPII Degradation Factor Def1 Is Required for Development, Stress Response, and Full Virulence ofJournal of fungi (Basel, Switzerland) · 2023Article
- Comprehensive Bioinformatics Analysis IdentifiesFrontiers in genetics · 2021Article
- Yeast Rpn4 Links the Proteasome and DNA Repair viaInternational journal of molecular sciences · 2020Article
- Functional interplay between Mediator and RNA polymerase II in Rad2/XPG loading to the chromatin.Nucleic acids research · 2019Article
- Rpb9-deficient cells are defective in DNA damage response and require histone H3 acetylation for survival.Scientific reports · 2018Article
- Stimulation of RNA Polymerase II ubiquitination and degradation by yeast mRNA 3'-end processing factors is a conserved DNA damage response in eukaryotes.DNA repair · 2017Article
- RNA Polymerase II Trigger Loop Mobility: INDIRECT EFFECTS OF Rpb9.The Journal of biological chemistry · 2016Article
- RNA polymerase II contributes to preventing transcription-mediated replication fork stalls.The EMBO journal · 2015Article
- Transcription bypass of DNA lesions enhances cell survival but attenuates transcription coupled DNA repair.Nucleic acids research · 2014Article
- The Not5 subunit of the ccr4-not complex connects transcription and translation.PLoS genetics · 2014Article
- Degradation of DNA damage-independently stalled RNA polymerase II is independent of the E3 ligase Elc1.Nucleic acids research · 2014Article
- Role of p97/VCP (Cdc48) in genome stability.Frontiers in genetics · 2013Article
- Diverse roles of RNA polymerase II-associated factor 1 complex in different subpathways of nucleotide excision repair.The Journal of biological chemistry · 2011Article
- Cdc48/p97 mediates UV-dependent turnover of RNA Pol II.Molecular cell · 2011Article
- The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair.The Journal of biological chemistry · 2010Article
- Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair.PLoS genetics · 2009Article
- Protein modifications in transcription elongation.Biochimica et biophysica acta · 2009Review
- Article
- Tfb5 is partially dispensable for Rad26 mediated transcription coupled nucleotide excision repair in yeast.DNA repair · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Rpb9, a nonessential subunit of RNA polymerase II (Pol II), has multiple transcription-related functions in Saccharomyces cerevisiae, including transcription elongation and transcription-coupled repair (TCR). Here we show that, in response to UV radiation, Rpb9 also functions in promoting ubiquitylation and degradation of Rpb1, the largest subunit of Pol II. This function of Rpb9 is not affected by any pathways of nucleotide excision repair, including TCR mediated by Rpb9 itself and by Rad26. Rpb9 is composed of three distinct domains: the N-terminal Zn1, the C-terminal Zn2, and the central linker. The Zn2 domain, which is dispensable for transcription elongation and TCR functions, is essential for Rpb9 to promote Rpb1 degradation, whereas the Zn1 and linker domains, which are essential for transcription elongation and TCR functions, play a subsidiary role in Rpb1 degradation. Coimmunoprecipitation analysis suggests that almost the full length of Rpb9 is required for a strong interaction with the core Pol II: deletion of the Zn2 domain causes dramatically weakened interaction, whereas deletion of Zn1 and the linker resulted in undetectable interaction. Furthermore, we show that Rpb1, rather than the whole Pol II complex, is degraded in response to UV radiation and that the degradation is primarily mediated by the 26S proteasome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.