ArticleMolecular and cellular biology2007
Epigenetic programming of the rRNA promoter by MBD3.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 60 citations in OpenAlex.
- Bridging MBD3 functions in developmental biology and glioma heterogeneity to immune remodeling with future perspectives.Frontiers in immunology · 2026Review
- It Takes a Village of Chromatin Remodelers to Regulate rDNA Expression.International journal of molecular sciences · 2025Review
- Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells.PloS one · 2024Article
- Modeling the consequences of age-linked rDNA hypermethylation with dCas9-directed DNA methylation in human cells.bioRxiv : the preprint server for biology · 2023Article
- Demethylation ofOncology letters · 2020Article
- Nucleolar expansion and elevated protein translation in premature aging.Nature communications · 2017Article
- Stress and the Emerging Roles of Chromatin Remodeling in Signal Integration and Stable Transmission of Reversible Phenotypes.Frontiers in behavioral neuroscience · 2017Review
- MBD3 mediates epigenetic regulation on EPAS1 promoter in cancer.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Article
- Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy.Nucleic acids research · 2015Article
- Basic mechanisms in RNA polymerase I transcription of the ribosomal RNA genes.Sub-cellular biochemistry · 2013Review
- Article
- DNA methylation, the early-life social environment and behavioral disorders.Journal of neurodevelopmental disorders · 2011Article
- The early life social environment and DNA methylation: DNA methylation mediating the long-term impact of social environments early in life.Epigenetics · 2011Review
- The chromatin remodelling complex B-WICH changes the chromatin structure and recruits histone acetyl-transferases to active rRNA genes.PloS one · 2011Article
- Child health, developmental plasticity, and epigenetic programming.Endocrine reviews · 2011Review
- The implications of DNA methylation for toxicology: toward toxicomethylomics, the toxicology of DNA methylation.Toxicological sciences : an official journal of the Society of Toxicology · 2011Article
- Article
- Methylation of histone H3 and H4 by PRMT5 regulates ribosomal RNA gene transcription.Journal of cellular biochemistry · 2010Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Within the human genome there are hundreds of copies of the rRNA gene, but only a fraction of these genes are active. Silencing through epigenetics has been extensively studied; however, it is essential to understand how active rRNA genes are maintained. Here, we propose a role for the methyl-CpG binding domain protein MBD3 in epigenetically maintaining active rRNA promoters. We show that MBD3 is localized to the nucleolus, colocalizes with upstream binding factor, and binds to unmethylated rRNA promoters. Knockdown of MBD3 by small interfering RNA results in increased methylation of the rRNA promoter coupled with a decrease in RNA polymerase I binding and pre-rRNA transcription. Conversely, overexpression of MBD3 results in decreased methylation of the rRNA promoter. Additionally, overexpression of MBD3 induces demethylation of nonreplicating plasmids containing the rRNA promoter. We demonstrate that this demethylation occurs following the overexpression of MBD3 and its increased interaction with the methylated rRNA promoter. This is the first demonstration that MBD3 is involved in inducing and maintaining the demethylated state of a specific promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.