ArticleMolecular and cellular biology2007
Linear derivatives of Saccharomyces cerevisiae chromosome III can be maintained in the absence of autonomously replicating sequence elements.
Article in Molecular and cellular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 44 citations in OpenAlex.
- Loss of G1-phase CDK-inhibition biases instability between genomic regions by unevenly reducing activity among replication origins.iScience · 2025Article
- Origins of DNA replication in eukaryotes.Molecular cell · 2023Review
- Review
- Genome-wide Cas9 binding specificity inPeerJ · 2020Article
- Phenotypic and Genotypic Consequences of CRISPR/Cas9 Editing of the Replication Origins in the rDNA ofGenetics · 2019Article
- Autonomously Replicating Linear Plasmids That Facilitate the Analysis of Replication Origin Function inmSphere · 2019Article
- Comprehensive Analysis of Replication Origins inFrontiers in microbiology · 2019Article
- The evolution of the temporal program of genome replication.Nature communications · 2018Article
- Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex inGenetics · 2017Article
- Order from clutter: selective interactions at mammalian replication origins.Nature reviews. Genetics · 2017Review
- Regulation of Replication Origins.Advances in experimental medicine and biology · 2017Review
- Eukaryotic replication origins: Strength in flexibility.Nucleus (Austin, Tex.) · 2016Review
- Hst3p, a histone deacetylase, promotes maintenance of Saccharomyces cerevisiae chromosome III lacking efficient replication origins.Molecular genetics and genomics : MGG · 2016Article
- How MCM loading and spreading specify eukaryotic DNA replication initiation sites.F1000Research · 2016Review
- Post-licensing Specification of Eukaryotic Replication Origins by Facilitated Mcm2-7 Sliding along DNA.Molecular cell · 2015Article
- Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe.Genome biology · 2015Article
- The DNA repair endonuclease Mus81 facilitates fast DNA replication in the absence of exogenous damage.Nature communications · 2015Article
- Developmental regulation of the Tetrahymena thermophila origin recognition complex.PLoS genetics · 2015Article
- The CDK regulators Cdh1 and Sic1 promote efficient usage of DNA replication origins to prevent chromosomal instability at a chromosome arm.Nucleic acids research · 2014Article
- Origin plasticity during budding yeast DNA replication in vitro.The EMBO journal · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Replication origins in Saccharomyces cerevisiae are spaced at intervals of approximately 40 kb. However, both measurements of replication fork rate and studies of hypomorphic alleles of genes encoding replication initiation proteins suggest the question of whether replication origins are more closely spaced than should be required. We approached this question by systematically deleting replicators from chromosome III. The first significant increase in loss rate detected for the 315-kb full-length chromosome occurred only after all five efficient chromosomal replicators in the left two-thirds of the chromosome (ARS305, ARS306, ARS307, ARS309, and ARS310) had been deleted. The removal of the inefficient replicator ARS308 from this originless region caused little or no additional increase in loss rate. Chromosome fragmentations that removed the normally inactive replicators on the left end of the chromosome or the replicators distal to ARS310 on the right arm showed that both groups of replicators contribute significantly to the maintenance of the originless chromosome. Surprisingly, a 142-kb derivative of chromosome III, lacking all sequences that function as autonomously replicating sequence elements in plasmids, replicated and segregated properly 97% of the time. Both the replication initiation protein ORC and telomeres or a linear topology were required for the maintenance of chromosome fragments lacking replicators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.