ArticleNucleic acids research2007
Genomewide and biochemical analyses of DNA-binding activity of Cdc6/Orc1 and Mcm proteins in Pyrococcus sp.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Archaeal DNA replication initiation: bridging LUCA's legacy and modern mechanisms.Frontiers in microbiology · 2025Article
- The Archaeal Cell Cycle.Annual review of cell and developmental biology · 2024Review
- Article
- Dissection of Functional Domains of Orc1-2, the Archaeal Global DNA Damage-Responsive Regulator.International journal of molecular sciences · 2022Article
- Diversity of DNA Replication in the Archaea.Genes · 2017Review
- Genome Replication inFrontiers in microbiology · 2017Article
- High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii.Scientific reports · 2016Article
- Comparative genomics reveals conserved positioning of essential genomic clusters in highly rearranged Thermococcales chromosomes.Biochimie · 2015Article
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Activation of the MCM helicase from the thermophilic archaeon, Thermoplasma acidophilum by interactions with GINS and Cdc6-2.Extremophiles : life under extreme conditions · 2014Article
- Genome Sequence of a Hyperthermophilic Archaeon, Thermococcus nautili 30-1, That Produces Viral Vesicles.Genome announcements · 2014Article
- Diversity of the DNA replication system in the Archaea domain.Archaea (Vancouver, B.C.) · 2014Review
- Characterization of the replication initiator Orc1/Cdc6 from the Archaeon Picrophilus torridus.Journal of bacteriology · 2014Article
- Article
- Defining components of the chromosomal origin of replication of the hyperthermophilic archaeon Pyrococcus furiosus needed for construction of a stable replicating shuttle vector.Applied and environmental microbiology · 2011Article
- The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication.Extremophiles : life under extreme conditions · 2011Article
- Hot transcriptomics.Archaea (Vancouver, B.C.) · 2011Article
- Localized melting of duplex DNA by Cdc6/Orc1 at the DNA replication origin in the hyperthermophilic archaeon Pyrococcus furiosus.Extremophiles : life under extreme conditions · 2010Article
- Quantitative analysis of replication-related mutation and selection pressures in bacterial chromosomes and plasmids using generalised GC skew index.BMC genomics · 2009Article
- Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The origin of DNA replication (oriC) of the hyperthermophilic archaeon Pyrococcus abyssi contains multiple ORB and mini-ORB repeats that show sequence similarities to other archaeal ORB (origin recognition box). We report here that the binding of Cdc6/Orc1 to a 5 kb region containing oriC in vivo was highly specific both in exponential and stationary phases, by means of chromatin immunoprecipitation coupled with hybridization on a whole genome microarray (ChIP-chip). The oriC region is practically the sole binding site for the Cdc6/Orc1, thereby distinguishing oriC in the 1.8 M bp genome. We found that the 5 kb region contains a previously unnoticed cluster of ORB and mini-ORB repeats in the gene encoding the small subunit (dp1) for DNA polymerase II (PolD). ChIP and the gel retardation analyses further revealed that Cdc6/Orc1 specifically binds both of the ORB clusters in oriC and dp1. The organization of the ORB clusters in the dp1 and oriC is conserved during evolution in the order Thermococcales, suggesting a role in the initiation of DNA replication. Our ChIP-chip analysis also revealed that Mcm alters the binding specificity to the oriC region according to the growth phase, consistent with its role as a licensing factor.
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