ArticleBiochimica et biophysica acta2007
Physical properties of the lipid bilayer membrane made of calf lens lipids: EPR spin labeling studies.
Article in Biochimica et biophysica acta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 53 citations in OpenAlex.
- Mechanical Properties of Eye Lens Cortical and Nuclear Membranes and the Whole Lens.Investigative ophthalmology & visual science · 2025Article
- 5-PC as a Lipid Probe Molecule and as a Second Phospholipid in Binary Phospholipid Mixtures: Saturation Recovery EPR Studies.International journal of molecular sciences · 2024Article
- Article
- Molecular oxygen as a probe molecule in EPR spin-labeling studies of membrane structure and dynamics.Oxygen (Basel, Switzerland) · 2022Article
- Multilamellar Liposomes as a Model for Biological Membranes: Saturation Recovery EPR Spin-Labeling Studies.Membranes · 2022Review
- Atomistic simulations modify interpretation of spin-label oximetry data. Part 1: intensified water-lipid interfacial resistances.Applied magnetic resonance · 2021Article
- Factors determining barrier properties to oxygen transport across model and cell plasma membranes based on EPR spin-label oximetry.Applied magnetic resonance · 2021Article
- AAngewandte Chemie (International ed. in English) · 2020Article
- Confocal Microscopy Confirmed that in Phosphatidylcholine Giant Unilamellar Vesicles with very High Cholesterol Content Pure Cholesterol Bilayer Domains Form.Cell biochemistry and biophysics · 2019Article
- Is the cholesterol bilayer domain a barrier to oxygen transport into the eye lens?Biochimica et biophysica acta. Biomembranes · 2018Article
- Cholesterol Bilayer Domains in the Eye Lens Health: A Review.Cell biochemistry and biophysics · 2017Review
- Whales, lifespan, phospholipids, and cataracts.Journal of lipid research · 2017Article
- Non-alcoholic fatty liver disease, to struggle with the strangle: Oxygen availability in fatty livers.Redox biology · 2017Review
- Influence of Cholesterol on the Oxygen Permeability of Membranes: Insight from Atomistic Simulations.Biophysical journal · 2017Article
- Organization of lipids in fiber-cell plasma membranes of the eye lens.Experimental eye research · 2017Review
- Lipid domains in intact fiber-cell plasma membranes isolated from cortical and nuclear regions of human eye lenses of donors from different age groups.Experimental eye research · 2015Article
- Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61-70-year-old human donors.European biophysics journal : EBJ · 2015Article
- Spin-label W-band EPR with seven-loop-six-gap resonator: Application to lens membranes derived from eyes of a single donor.Applied magnetic resonance · 2014Article
- Lipid-protein interactions in plasma membranes of fiber cells isolated from the human eye lens.Experimental eye research · 2014Article
- Measuring the viscosity of whole bovine lens using a fiber optic oxygen sensing system.Molecular vision · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The physical properties of a membrane derived from the total lipids of a calf lens were investigated using EPR spin labeling and were compared with the properties of membranes made of an equimolar 1-palmitoyl-2-oleoylphosphatidylcholine/cholesterol (POPC/Chol) mixture and of pure POPC. Conventional EPR spectra and saturation-recovery curves show that spin labels detect a single homogenous environment in all three membranes. Profiles of the order parameter, hydrophobicity, and oxygen transport parameter are practically identical in lens lipid and POPC/Chol membranes, but differ drastically from profiles in pure POPC membranes. In both lens lipid and POPC/Chol membranes, the lipids are strongly immobilized at all depths, which is in contrast to the high fluidity of the POPC membrane. Hydrophobicity and oxygen transport parameter profiles in lens lipid and POPC/Chol membranes have a rectangular shape with an abrupt change between the C9 and C10 positions, which is approximately where the steroid ring structure of cholesterol reaches into the membrane. At this position, hydrophobicity increases from the level of methanol to the level of hexane, and the oxygen transport parameter increases by a factor of 2-3. These profiles in POPC membranes are bell-shaped. It is concluded that the high level of cholesterol in lens lipids makes the membrane stable, immobile, and impermeable to both polar and nonpolar molecules.
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