ArticleJournal of anatomy2007
Human mesenchymal stem cells at the single-cell level: simultaneous seven-colour immunofluorescence.
Article in Journal of anatomy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Article
- Enhanced Transfection of Human Mesenchymal Stem Cells Using a Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System.Polymers · 2019Article
- Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis.PloS one · 2019Article
- Crohn's disease patient serum changes protein expression in a human mesenchymal stem cell model in a linear relationship to patients' disease stage and to bone mineral density.Journal of clinical & translational endocrinology · 2018Article
- Decellularized Wharton's Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications.PloS one · 2017Article
- Adult Human Mesenchymal Stem Cell Differentiation at the Cell Population and Single-Cell Levels Under Alternating Electric Current.Tissue engineering. Part C, Methods · 2016Article
- Choice of Illumination System & Fluorophore for Multiplex Immunofluorescence on FFPE Tissue Sections.PloS one · 2016Article
- Rat visceral yolk sac cells: viability and expression of cell markers during maternal diabetes.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Article
- Continuous-flow sorting of stem cells and differentiation products based on dielectrophoresis.Lab on a chip · 2015Article
- Filter-Dense Multicolor Microscopy.PloS one · 2015Article
- Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes.Chembiochem : a European journal of chemical biology · 2012Article
- Multiplexed in situ immunofluorescence using dynamic DNA complexes.Angewandte Chemie (International ed. in English) · 2012Article
- Quantitative comparison between full-spectrum and filter-based imaging in hyperspectral fluorescence microscopy.Journal of microscopy · 2012Article
- The transcriptional coactivator TAZ regulates mesenchymal differentiation in malignant glioma.Genes & development · 2011Article
- Impact of indium-111 oxine labelling on viability of human mesenchymal stem cells in vitro, and 3D cell-tracking using SPECT/CT in vivo.Molecular imaging and biology · 2011Article
- Characterization of eight different tetracyclines: advances in fluorescence bone labeling.Journal of anatomy · 2010Article
- Morphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing.Journal of anatomy · 2009Article
- Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy.Journal of cellular and molecular medicine · 2008Article
- Phenotypic Characterization of Mesenchymal Stem Cells from Various Tissues.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2008Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Extracellular, intracellular or surface proteins can be used as putative markers to characterize human mesenchymal stem cells (hMSC). However, these markers are also expressed by other cell types and primary cell pools reveal considerable heterogeneity. Therefore, the simultaneous detection of several markers on a single cell appears to be an attractive approach to identify hMSC. Here we demonstrate the specific distinction of human MSC from human osteoblasts via seven-colour fluorescence on the single cell level with simultaneous marker detection of CD44, CD105/endoglin, CD106/VCAM-1, collagen-IV, fibronectin, actin and DAPI nuclear staining. We performed spectral image acquisition using a Sagnac-type interferometer. Subsequent linear unmixing allowed for decomposition of each pixel in its spectral components. Our approach reveals a typical expression profile of the adherent singular cells, allowing the specific distinction between hMSC and osteoblasts on the single cell level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.