Evidence map›Paper›PMID 17450148›Full record

ArticleNature methods2007

A yeast two-hybrid smart-pool-array system for protein-interaction mapping.

Fulai Jin, Larisa Avramova, Jing Huang, Tony Hazbun

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012
    Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Fulai JinDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, and the Molecular Biology Institute, University of California, Los Angeles, California 90095, USA.
Larisa Avramova
Jing Huang
Tony Hazbun
Purdue University West Lafayette · USUniversity of California, Los Angeles · US

Funding

Exploiting TOR Signaling for Cancer Drug DiscoveryR01CA124974 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HUANG, JING · 2007 to 2011
$1.5M
A Mixed-Bait-Strategy for Protein Interactome MappingR21HG003729 · NHGRI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI HUANG, JING · 2006 to 2007
$280k
NCI NIH HHS R01 CA124974NHGRI NIH HHS HG003729NHGRI NIH HHS R21 HG003729
6 · The paper itself

Abstract

We present here a new two-hybrid smart pool array (SPA) system in which, instead of individual activation domain strains, well-designed activation domain pools are screened in an array format that allows built-in replication and prey-bait deconvolution. Using this method, a Saccharomyces cerevisiae genome SPA increases yeast two-hybrid screening efficiency by an order of magnitude.

Indexed as

Two-Hybrid System TechniquesGenome, FungalProtein Interaction MappingSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSaccharomyces cerevisiae Proteins

Identifiers

PMID17450148
PMCPMC2771206
OpenAlexW2008295579

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.